Detailed information of TA system TA178728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 88461..89218
• Replicon: plasmid pKP19-3023-374k
• Accession: NZ_CP063748
• Organism: Klebsiella pneumoniae strain KP19-3023

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J4ZXV7
• Locus tag: IMY55_RS26530
• Protein ID: WP_029497408.1
• Coordinates: 88736..89218 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: IMY55_RS26525
• Protein ID: WP_094645258.1
• Coordinates: 88461..88748 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMY55_RS26510	84634..86003	+	1370	WP_094645257.1	IS3 family transposase	-
IMY55_RS26515	86529..87101	+	573	WP_094645259.1	cytochrome b/b6 domain-containing protein	-
IMY55_RS26520	87301..88224	+	924	WP_000167917.1	cation diffusion facilitator family transporter	-
IMY55_RS26525	88461..88748	+	288	WP_094645258.1	DUF1778 domain-containing protein	Antitoxin
IMY55_RS26530	88736..89218	+	483	WP_029497408.1	GNAT family N-acetyltransferase	Toxin
IMY55_RS26535	89557..89718	+	162	WP_017900895.1	type I toxin-antitoxin system Hok family toxin	-
IMY55_RS26540	89926..90186	-	261	WP_009653224.1	hypothetical protein	-
IMY55_RS26545	90726..91697	+	972	WP_009653249.1	hypothetical protein	-
IMY55_RS26550	92060..92437	-	378	WP_042927811.1	hypothetical protein	-
IMY55_RS26555	93972..94187	+	216	WP_009653220.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / blaNDM-1 / qacE / qnrB6 / aadA16 / dfrA27 / ARR-3	katA	1..374513	374513
	-	flank	IS/Tn	-	-	85200..86003	803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17599.34 Da
• Isoelectric Point: 9.8719

>T178728 WP_029497408.1 NZ_CP063748:88736-89218 [Klebsiella pneumoniae]
VGRLTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLRLP

Nucleotide

• Length: 483 bp

>T178728 NZ_CP084222:c962154-961831 [Arsenophonus apicola]
ATGGAATACCTACTTTTTATTGAAACCCCTGTATTTAGTAGAGAACGTACAGAACTGTTAACCGATGATGAATTTAGACA
ACTACAACAATACTTACTAAAAAATCATGAACAAGGCAGTACCATTAGCGCTACTGGAGGATGTAAAAAGATCCGTTGGT
TGAGGGAAGGAATGGGAAAACAGGGCGGTGTCAGAATCATTTACTACACCATGACGAAAATTGGAAAGATCTATCTTCTT
TTAATCTACCCAAAAAATAAAAAAGATGACTTAACCTCAAAAGAAAAAGAAGTACTTAAAGCCATCATTAATAAATTGCA
ATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10955.66 Da
• Isoelectric Point: 4.5141

>AT178728 WP_094645258.1 NZ_CP063748:88461-88748 [Klebsiella pneumoniae]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVVDD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT178728 NZ_CP084222:c961784-961488 [Arsenophonus apicola]
ATGGACAAAAAACTTTTTGGCGATCTGGTTGAAAGCATGAATCAGATGGTAGCGATGGAAAAAGGGGAACGTTCTATTCC
TTCTGAAAATATTCATCGCCATCGTCTGCCTAATGTAAAAAACCTACGTGAAACCTTTGGCTTAAAGCAAAGTGAATTTG
CCGAAGCTGTTGGCGTTTCTGCATCCTTAATTCAGAGCTGGGAACAACACCGCCGTATTCCTTCTGGTTCAGCACTTAAA
TTACTTAAAATGCTTGAGCGTAATCCAACGCTAATTAATGAGCTAAGTACACTTTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZXV7