Detailed information of TA system TA178709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2647034..2647563
• Replicon: chromosome
• Accession: NZ_CP063745
• Organism: Staphylococcus aureus strain NAS_AN_212

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF732_RS12885
• Protein ID: WP_000621175.1
• Coordinates: 2647201..2647563 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF732_RS12880
• Protein ID: WP_000948331.1
• Coordinates: 2647034..2647204 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF732_RS12850 (2642070)	2642070..2642630	+	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-
IF732_RS12855 (2642839)	2642839..2643318	+	480	WP_240104851.1	hypothetical protein	-
IF732_RS12860 (2643311)	2643311..2644888	+	1578	WP_001294650.1	PH domain-containing protein	-
IF732_RS12865 (2644881)	2644881..2645372	+	492	WP_001286980.1	PH domain-containing protein	-
IF732_RS12870 (2645376)	2645376..2645735	+	360	WP_000581197.1	holo-ACP synthase	-
IF732_RS12875 (2645801)	2645801..2646949	+	1149	WP_001281139.1	alanine racemase	-
IF732_RS12880 (2647034)	2647034..2647204	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF732_RS12885 (2647201)	2647201..2647563	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF732_RS12890 (2647913)	2647913..2648914	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF732_RS12895 (2649033)	2649033..2649359	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF732_RS12900 (2649361)	2649361..2649840	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
IF732_RS12905 (2649815)	2649815..2650585	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T178709 WP_000621175.1 NZ_CP063745:2647201-2647563 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T178709 NZ_CP084212:c1952524-1952240 [Pseudomonas amygdali]
ATGCAGGTTGAGTGGCTTAAAACGGCACTAAAGAATCTGGATGAGGAGGCAGCGTATATCGCACAGGACAATCCAGCTGC
TGCCGCAGCTTTCGTGAAAGCAATTCAATCAAGCGTGACGCAGCTCGCGAGCTTTCCCGCCATGGGCCGGGAAGGACGGA
TTGCCGGAGCCAGAGAATGGCCTCTGCCAGACCTGCCTTACCTGATTCCATATCGCATCCGTAGCGGCCGGCTACAGGTT
CTAAGGATTTTCCACACCAGAAGACAATCGCCCCCCGTGTGGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT178709 WP_000948331.1 NZ_CP063745:2647034-2647204 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT178709 NZ_CP084212:c1952753-1952514 [Pseudomonas amygdali]
ATGTCCACAATGTCTCTACGTCTGCCGGACGAAATGGCGGATACCCTTGCACACCTTGCAAAGGCGACCGGGCGCAGCAA
GTCATTTTTGGCCCTCGACGCGCTCCGCGAATACCTGACTCGCGAGGCATGGCAGATTGCCGAAATTCAGCGCGCGATTG
AAGAAGCCGACGCTGGCGAGTTCGCCAGTACAGAGGACGTAAAAGCTGTCATGGACAAATGGTCTGGCAATGCAGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1