Detailed information of TA system TA178701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1149992..1150468
• Replicon: chromosome
• Accession: NZ_CP063745
• Organism: Staphylococcus aureus strain NAS_AN_212

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF732_RS05665
• Protein ID: WP_000052076.1
• Coordinates: 1150280..1150468 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF732_RS05660
• Protein ID: WP_001059690.1
• Coordinates: 1149992..1150252 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF732_RS05625 (1145390)	1145390..1146106	-	717	WP_000150026.1	type I 3-dehydroquinate dehydratase	-
IF732_RS05630 (1146254)	1146254..1146676	+	423	WP_000974452.1	organic hydroperoxide resistance protein	-
IF732_RS05635 (1147075)	1147075..1147569	+	495	WP_000569881.1	GNAT family N-acetyltransferase	-
IF732_RS05640 (1147718)	1147718..1148335	+	618	WP_000255562.1	LysE/ArgO family amino acid transporter	-
IF732_RS05645 (1148408)	1148408..1148989	-	582	WP_001140696.1	histidine phosphatase family protein	-
IF732_RS05650 (1149214)	1149214..1149450	+	237	WP_000368687.1	hypothetical protein	-
IF732_RS05655 (1149447)	1149447..1149650	-	204	WP_000145166.1	sterile alpha motif-like domain-containing protein	-
IF732_RS05660 (1149992)	1149992..1150252	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF732_RS05665 (1150280)	1150280..1150468	+	189	WP_000052076.1	hypothetical protein	Toxin
IF732_RS05670 (1150656)	1150656..1151225	+	570	WP_001106655.1	lipoprotein N-acylation protein LnsA	-
IF732_RS05675 (1151313)	1151313..1151597	+	285	WP_001004403.1	hypothetical protein	-
IF732_RS05680 (1151658)	1151658..1151876	+	219	WP_000005881.1	hypothetical protein	-
IF732_RS05685 (1152373)	1152373..1152573	-	201	WP_001059082.1	cold-shock protein	-
IF732_RS05690 (1152930)	1152930..1153616	-	687	WP_001574556.1	thermonuclease family protein	-
IF732_RS05695 (1153957)	1153957..1154439	-	483	WP_000824490.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T178701 WP_000052076.1 NZ_CP063745:1150280-1150468 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T178701 NZ_CP084209:c3588948-3588550 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT178701 WP_001059690.1 NZ_CP063745:1149992-1150252 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT178701 NZ_CP084209:c3589244-3588951 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56