Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1179223..1179860 Replicon chromosome
Accession NZ_CP063687
Organism Bacillus velezensis strain NST6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BACVE_RS05935 Protein ID WP_003156187.1
Coordinates 1179223..1179573 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BACVE_RS05940 Protein ID WP_003156188.1
Coordinates 1179579..1179860 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BACVE_RS05895 1174263..1174865 - 603 WP_007410234.1 SpoIIE family protein phosphatase -
BACVE_RS05900 1174865..1175653 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BACVE_RS05905 1175619..1176101 - 483 WP_003156173.1 anti-sigma B factor RsbW -
BACVE_RS05910 1176098..1176427 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BACVE_RS05915 1176491..1177498 - 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
BACVE_RS05920 1177510..1177911 - 402 WP_017419223.1 anti-sigma regulatory factor -
BACVE_RS05925 1177914..1178279 - 366 WP_007609584.1 RsbT antagonist protein RsbS -
BACVE_RS05930 1178284..1179105 - 822 WP_003156182.1 STAS domain-containing protein -
BACVE_RS05935 1179223..1179573 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BACVE_RS05940 1179579..1179860 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BACVE_RS05945 1179980..1181149 - 1170 WP_065180972.1 alanine racemase -
BACVE_RS05950 1181266..1182273 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BACVE_RS05955 1182438..1182803 - 366 WP_007609580.1 holo-ACP synthase -
BACVE_RS05960 1182896..1183495 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T178692 WP_003156187.1 NZ_CP063687:c1179573-1179223 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T178692 NZ_CP084209:1956267-1956370 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT178692 WP_003156188.1 NZ_CP063687:c1179860-1179579 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT178692 NZ_CP084209:c1956408-1956137 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References