Detailed information of TA system TA178691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 363239..364156
• Replicon: chromosome
• Accession: NZ_CP063687
• Organism: Bacillus velezensis strain NST6

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BACVE_RS01600
• Protein ID: WP_007407256.1
• Coordinates: 363239..363985 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BACVE_RS01605
• Protein ID: WP_003154807.1
• Coordinates: 363986..364156 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BACVE_RS01580	358632..359582	-	951	WP_007610844.1	ring-cleaving dioxygenase	-
BACVE_RS01585	359904..361220	+	1317	WP_063636817.1	amino acid permease	-
BACVE_RS01590	361507..362124	+	618	WP_003154804.1	DUF47 domain-containing protein	-
BACVE_RS01595	362137..363135	+	999	WP_017417610.1	inorganic phosphate transporter	-
BACVE_RS01600	363239..363985	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BACVE_RS01605	363986..364156	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BACVE_RS01610	364413..365291	-	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BACVE_RS01615	365305..365568	-	264	WP_003154813.1	phage holin	-
BACVE_RS01620	365582..365845	-	264	WP_021493821.1	hemolysin XhlA family protein	-
BACVE_RS01625	365897..366658	-	762	WP_063636818.1	hypothetical protein	-
BACVE_RS01630	366715..366912	-	198	WP_007610833.1	XkdX family protein	-
BACVE_RS01635	366917..367288	-	372	WP_038457736.1	YomQ/XkdW protein, phage-like element PBSX	-
BACVE_RS01640	367301..367768	-	468	WP_076984018.1	hypothetical protein	-
BACVE_RS01645	367666..368325	-	660	WP_017417606.1	terminase	-
BACVE_RS01650	368438..368950	-	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T178691 WP_007407256.1 NZ_CP063687:363239-363985 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T178691 NZ_CP084209:c1802681-1802307 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT178691 WP_003154807.1 NZ_CP063687:363986-364156 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT178691 NZ_CP084209:c1803138-1802770 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14