Detailed information of TA system TA178682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 2210828..2211387
• Replicon: chromosome
• Accession: NZ_CP063659
• Organism: Synechocystis sp. PCC 7339

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IQE94_RS10515
• Protein ID: WP_190599655.1
• Coordinates: 2211049..2211387 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IQE94_RS10510
• Protein ID: WP_212718063.1
• Coordinates: 2210828..2211052 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQE94_RS10490 (IQE94_10390)	2206466..2207032	-	567	WP_223341874.1	5-formyltetrahydrofolate cyclo-ligase	-
IQE94_RS10495 (IQE94_10395)	2207193..2207945	+	753	WP_223341875.1	HAD family hydrolase	-
IQE94_RS10500 (IQE94_10400)	2208050..2209252	+	1203	WP_223341876.1	class I SAM-dependent methyltransferase	-
IQE94_RS10505 (IQE94_10405)	2209265..2210668	-	1404	WP_212718060.1	glutamate decarboxylase	-
IQE94_RS10510 (IQE94_10410)	2210828..2211052	+	225	WP_212718063.1	DUF433 domain-containing protein	Antitoxin
IQE94_RS10515 (IQE94_10415)	2211049..2211387	+	339	WP_190599655.1	DUF5615 family PIN-like protein	Toxin
IQE94_RS10520 (IQE94_10420)	2211511..2212479	-	969	WP_212718064.1	carbon-nitrogen hydrolase family protein	-
IQE94_RS10525 (IQE94_10425)	2212507..2213313	-	807	WP_212718065.1	urease accessory protein UreD	-
IQE94_RS10530 (IQE94_10430)	2213306..2213488	+	183	WP_223341877.1	hypothetical protein	-
IQE94_RS10535 (IQE94_10435)	2213576..2214025	-	450	WP_223341878.1	photosystem II protein PsbQ	-
IQE94_RS10540 (IQE94_10440)	2214332..2215228	+	897	WP_212718068.1	prohibitin family protein	-
IQE94_RS10545 (IQE94_10445)	2215338..2215859	-	522	WP_212718069.1	gamma carbonic anhydrase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-78)

Antitoxin

• Protein of unknown function DUF433 (9-63)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12438.38 Da
• Isoelectric Point: 4.3410

>T178682 WP_190599655.1 NZ_CP063659:2211049-2211387 [Synechocystis sp. PCC 7339]
MNIWVDAQLPPTLADWLSSSFDLEAAALRDLGLRDAKDIEIFDAARVANSVIMTKDSDFVDLVCRLGIPPQIIWLTCGNV
TNRNLRRILSSTLPKALEKLQEGEMIVEISSP

Nucleotide

• Length: 339 bp

>T178682 NZ_CP084209:c608432-607968 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8194.48 Da
• Isoelectric Point: 4.4013

>AT178682 WP_212718063.1 NZ_CP063659:2210828-2211052 [Synechocystis sp. PCC 7339]
MNSLLSRVTQIPGQCGGRPCIRGMRIRVSDILEMLAENVSTSEILEDFPDLEIADIQACLVFAARRTDFPQLTA

Nucleotide

• Length: 225 bp

>AT178682 NZ_CP084209:c608767-608432 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA