Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4382079..4382710 Replicon chromosome
Accession NZ_CP063518
Organism Escherichia coli strain EH01-18-02

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag IQS16_RS21080 Protein ID WP_001259388.1
Coordinates 4382435..4382710 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IQS16_RS21075 Protein ID WP_000593555.1
Coordinates 4382079..4382438 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS16_RS21050 4378211..4379155 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
IQS16_RS21055 4379152..4379985 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
IQS16_RS21060 4379985..4380749 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
IQS16_RS21065 4380746..4381552 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
IQS16_RS21070 4381558..4381959 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
IQS16_RS21075 4382079..4382438 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IQS16_RS21080 4382435..4382710 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
IQS16_RS21085 4382783..4383907 - 1125 WP_001314210.1 ABC transporter permease -
IQS16_RS21090 4383907..4386642 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
IQS16_RS21095 4386639..4387706 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T178674 WP_001259388.1 NZ_CP063518:c4382710-4382435 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T178674 NZ_CP084207:3418904-3419011 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT178674 WP_000593555.1 NZ_CP063518:c4382438-4382079 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT178674 NZ_CP084207:c3418854-3418791 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References