Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3745916..3746570 Replicon chromosome
Accession NZ_CP063518
Organism Escherichia coli strain EH01-18-02

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag IQS16_RS17915 Protein ID WP_000244772.1
Coordinates 3745916..3746323 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS16_RS17920 Protein ID WP_000354046.1
Coordinates 3746304..3746570 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS16_RS17895 3741873..3743606 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
IQS16_RS17900 3743612..3744322 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS16_RS17905 3744347..3745243 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IQS16_RS17910 3745355..3745876 + 522 WP_001055874.1 flavodoxin FldB -
IQS16_RS17915 3745916..3746323 - 408 WP_000244772.1 protein YgfX Toxin
IQS16_RS17920 3746304..3746570 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS16_RS17925 3746813..3747793 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IQS16_RS17930 3747870..3748529 - 660 WP_000250274.1 hemolysin III family protein -
IQS16_RS17935 3748693..3749004 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IQS16_RS17940 3749049..3750482 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
IQS16_RS17945 3750539..3751282 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T178671 WP_000244772.1 NZ_CP063518:c3746323-3745916 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178671 NZ_CP084207:c3179315-3179139 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178671 WP_000354046.1 NZ_CP063518:c3746570-3746304 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178671 NZ_CP084207:c3179093-3178677 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References