Detailed information of TA system TA178669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3313557..3314182
• Replicon: chromosome
• Accession: NZ_CP063518
• Organism: Escherichia coli strain EH01-18-02

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IQS16_RS15855
• Protein ID: WP_000911330.1
• Coordinates: 3313557..3313955 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IQS16_RS15860
• Protein ID: WP_000450524.1
• Coordinates: 3313955..3314182 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS16_RS15835	3309464..3309664	+	201	WP_000383836.1	YpfN family protein	-
IQS16_RS15840	3309745..3310443	-	699	WP_000679812.1	esterase	-
IQS16_RS15845	3310517..3312532	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
IQS16_RS15850	3312547..3313410	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
IQS16_RS15855	3313557..3313955	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IQS16_RS15860	3313955..3314182	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IQS16_RS15865	3314336..3315049	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IQS16_RS15870	3315262..3316296	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
IQS16_RS15875	3316313..3317191	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IQS16_RS15880	3317337..3317909	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IQS16_RS15885	3317909..3318379	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T178669 WP_000911330.1 NZ_CP063518:c3313955-3313557 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T178669 NZ_CP084207:3042968-3043255 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT178669 WP_000450524.1 NZ_CP063518:c3314182-3313955 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT178669 NZ_CP084207:3042729-3042968 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33