Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1093467..1094304 Replicon chromosome
Accession NZ_CP063518
Organism Escherichia coli strain EH01-18-02

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag IQS16_RS05130 Protein ID WP_000227784.1
Coordinates 1093467..1094009 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag IQS16_RS05135 Protein ID WP_001297137.1
Coordinates 1093993..1094304 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS16_RS05110 1089006..1089917 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
IQS16_RS05115 1090085..1090576 + 492 WP_001138904.1 nucleotide binding protein YajQ -
IQS16_RS05120 1090704..1092068 - 1365 WP_001000974.1 MFS transporter -
IQS16_RS05125 1092476..1093411 + 936 WP_001365761.1 sel1 repeat family protein -
IQS16_RS05130 1093467..1094009 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
IQS16_RS05135 1093993..1094304 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
IQS16_RS05140 1094489..1095379 - 891 WP_000971336.1 heme o synthase -
IQS16_RS05145 1095391..1095720 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
IQS16_RS05150 1095720..1096334 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
IQS16_RS05155 1096324..1098315 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
IQS16_RS05160 1098337..1099284 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T178653 WP_000227784.1 NZ_CP063518:c1094009-1093467 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T178653 NZ_CP084207:111032-111373 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT178653 WP_001297137.1 NZ_CP063518:c1094304-1093993 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT178653 NZ_CP084207:c111037-110961 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References