Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 96224..96825 Replicon plasmid pEH01-18-04-A_1
Accession NZ_CP063516
Organism Escherichia coli strain EH01-18-04-A

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag IQS17_RS25110 Protein ID WP_001216034.1
Coordinates 96224..96604 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IQS17_RS25115 Protein ID WP_001190712.1
Coordinates 96604..96825 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS17_RS25075 91275..92207 - 933 WP_000081352.1 homocysteine S-methyltransferase -
IQS17_RS25080 92194..93597 - 1404 WP_001373486.1 S-methylmethionine permease -
IQS17_RS25085 93805..94821 + 1017 WP_012372828.1 IS5-like element IS5 family transposase -
IQS17_RS25090 95049..95366 + 318 WP_001513659.1 hypothetical protein -
IQS17_RS25095 95316..95729 - 414 Protein_108 DDE-type integrase/transposase/recombinase -
IQS17_RS25100 95653..96012 - 360 WP_001513660.1 hypothetical protein -
IQS17_RS25105 96040..96219 - 180 WP_001513661.1 hypothetical protein -
IQS17_RS25110 96224..96604 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IQS17_RS25115 96604..96825 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IQS17_RS25120 97008..98564 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
IQS17_RS25125 98561..99844 + 1284 WP_001617890.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17 senB 1..149434 149434


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T178637 WP_001216034.1 NZ_CP063516:c96604-96224 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T178637 NZ_CP084205:2233827-2234114 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT178637 WP_001190712.1 NZ_CP063516:c96825-96604 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT178637 NZ_CP084205:2233588-2233827 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References