Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4304374..4304992 Replicon chromosome
Accession NZ_CP063515
Organism Escherichia coli strain EH01-18-04-A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IQS17_RS20830 Protein ID WP_001291435.1
Coordinates 4304374..4304592 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IQS17_RS20835 Protein ID WP_000344800.1
Coordinates 4304618..4304992 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS17_RS20795 4299662..4300234 + 573 WP_000779841.1 YbaY family lipoprotein -
IQS17_RS20800 4300265..4300576 - 312 WP_000409908.1 MGMT family protein -
IQS17_RS20810 4300955..4301308 + 354 WP_000878147.1 DUF1428 family protein -
IQS17_RS20815 4301350..4302900 - 1551 WP_001545832.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IQS17_RS20820 4303064..4303534 - 471 WP_000136192.1 YlaC family protein -
IQS17_RS20825 4303650..4304201 - 552 WP_000102539.1 maltose O-acetyltransferase -
IQS17_RS20830 4304374..4304592 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IQS17_RS20835 4304618..4304992 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IQS17_RS20840 4305538..4308687 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
IQS17_RS20845 4308710..4309903 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178631 WP_001291435.1 NZ_CP063515:c4304592-4304374 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T178631 NZ_CP084205:c1108315-1107986 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT178631 WP_000344800.1 NZ_CP063515:c4304992-4304618 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT178631 NZ_CP084205:c1108653-1108336 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References