Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3890907..3891457 Replicon chromosome
Accession NZ_CP063515
Organism Escherichia coli strain EH01-18-04-A

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag IQS17_RS18900 Protein ID WP_001160966.1
Coordinates 3891143..3891457 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag IQS17_RS18895 Protein ID WP_000125566.1
Coordinates 3890907..3891140 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS17_RS18870 3885980..3886267 + 288 WP_000203741.1 ferredoxin-like protein FixX -
IQS17_RS18875 3886326..3887657 + 1332 WP_001183207.1 MFS transporter -
IQS17_RS18880 3887765..3888295 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
IQS17_RS18885 3888288..3890150 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
IQS17_RS18890 3890342..3890821 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
IQS17_RS18895 3890907..3891140 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
IQS17_RS18900 3891143..3891457 + 315 WP_001160966.1 CcdB family protein Toxin
IQS17_RS18905 3891454..3892302 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
IQS17_RS18910 3892309..3892686 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
IQS17_RS18915 3892689..3893510 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
IQS17_RS18920 3893507..3894496 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
IQS17_RS18925 3894496..3895782 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T178629 WP_001160966.1 NZ_CP063515:3891143-3891457 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T178629 NZ_CP084205:844945-845352 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT178629 WP_000125566.1 NZ_CP063515:3890907-3891140 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT178629 NZ_CP084205:844698-844964 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References