Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2627121..2627921 Replicon chromosome
Accession NZ_CP063515
Organism Escherichia coli strain EH01-18-04-A

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag IQS17_RS12945 Protein ID WP_000342452.1
Coordinates 2627121..2627648 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag IQS17_RS12950 Protein ID WP_001277107.1
Coordinates 2627655..2627921 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS17_RS12920 2622197..2622964 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
IQS17_RS12925 2622961..2624238 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
IQS17_RS12930 2624235..2625161 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IQS17_RS12935 2625209..2626318 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
IQS17_RS12940 2626741..2627124 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
IQS17_RS12945 2627121..2627648 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
IQS17_RS12950 2627655..2627921 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
IQS17_RS12955 2628070..2629173 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
IQS17_RS12960 2629444..2630298 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
IQS17_RS12965 2630543..2631601 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
IQS17_RS12970 2631594..2632262 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T178619 WP_000342452.1 NZ_CP063515:c2627648-2627121 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T178619 NZ_CP084198:69568-69912 [Pantoea agglomerans]
ATGGACGCACTATTCATCGAACTTTCCAGCTTCCAGAAATACCGGGCTGATTATCTGAATGATGATCAGTACAGGCTGTT
TCAGAACATGTTGCTGGCTGACCCTGAGAAGGGCGATGTGATACCCGATACCGGCGGACTGCGTAAAGTTCGCTTCAGGG
ATGAGCGCCGACACAAGGGCGCACGTGGCGGGATCAGGGTTATCTATTACTGGACCAACACACAAGGCCAATTCATCCTG
TTTACCATCTACGATAAAGATCAACGCGACGATTTGACCAGGCAACAGCGTGATGCCCTGGCGACCGCGCTGAATGTGAT
CAAAAAAGGACTGAGACATGACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT178619 WP_001277107.1 NZ_CP063515:c2627921-2627655 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT178619 NZ_CP084198:69905-70219 [Pantoea agglomerans]
ATGACTGAACGCGATATCTTCAGTGAACTGATGACGGGTATGCAGGAGCTTAAGGATCATCAGGAGGGCAAAATTACGCT
TAAGAGTTATAAGGTCACTAAGCGTGCCCCTGTAACTATTGCGCCGCAGGAGTTACGCGACGTACGCGAAAAACTGAACC
TCTCACAGGCCGTATTCGCCCATTATCTTCATACTGGCGAAACGACGTATCAAAACTGGGAACAGGGCAGGGCGAGACCT
AACGCCCAGGCTGTTTTACTGATCCGCATGGTGCAGCAGAACCCAGAAACGCTGAAGACGCTCGCGAAGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References