Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 365170..366758 Replicon chromosome
Accession NZ_CP063515
Organism Escherichia coli strain EH01-18-04-A

Toxin (Protein)


Gene name hipA Uniprot ID A0A061YM43
Locus tag IQS17_RS01775 Protein ID WP_001125411.1
Coordinates 365170..366492 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A061YNW4
Locus tag IQS17_RS01780 Protein ID WP_001296076.1
Coordinates 366492..366758 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS17_RS01755 361848..362558 - 711 WP_001195177.1 fimbria/pilus periplasmic chaperone -
IQS17_RS01760 362920..363069 - 150 Protein_349 fimbrial protein -
IQS17_RS01765 363124..363821 + 698 Protein_350 IS1 family transposase -
IQS17_RS01770 363838..364260 - 423 Protein_351 fimbrial protein -
IQS17_RS01775 365170..366492 - 1323 WP_001125411.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
IQS17_RS01780 366492..366758 - 267 WP_001296076.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
IQS17_RS01785 367066..367824 + 759 WP_001286537.1 trans-aconitate 2-methyltransferase -
IQS17_RS01790 367828..368742 - 915 WP_001296077.1 bestrophin family protein -
IQS17_RS01795 368939..370390 - 1452 WP_000854638.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 349006..366758 17752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49329.96 Da        Isoelectric Point: 7.7906

>T178600 WP_001125411.1 NZ_CP063515:c366492-365170 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Download         Length: 1323 bp

>T178600 NZ_CP084194:2108171-2108274 [Salmonella sp. A39]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9987.34 Da        Isoelectric Point: 4.9632

>AT178600 WP_001296076.1 NZ_CP063515:c366758-366492 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Download         Length: 267 bp

>AT178600 NZ_CP084194:c2108276-2108131 [Salmonella sp. A39]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061YM43

Antitoxin

Source ID Structure
AlphaFold DB A0A061YNW4

References