Detailed information of TA system TA178594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3524725..3525363
• Replicon: chromosome
• Accession: NZ_CP063511
• Organism: Escherichia coli strain EH08-18-36

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: IQS18_RS17080
• Protein ID: WP_000813794.1
• Coordinates: 3525187..3525363 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IQS18_RS17075
• Protein ID: WP_001270286.1
• Coordinates: 3524725..3525141 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS18_RS17050	3520042..3521055	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
IQS18_RS17055	3521073..3522029	-	957	Protein_3314	ABC transporter substrate-binding protein	-
IQS18_RS17060	3522082..3522779	+	698	Protein_3315	IS1 family transposase	-
IQS18_RS17065	3522798..3522995	-	198	Protein_3316	spermidine/putrescine ABC transporter substrate-binding protein	-
IQS18_RS17070	3523240..3524646	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
IQS18_RS17075	3524725..3525141	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IQS18_RS17080	3525187..3525363	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IQS18_RS17085	3525585..3525815	+	231	WP_000494244.1	YncJ family protein	-
IQS18_RS17090	3525907..3527868	-	1962	WP_001752477.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IQS18_RS17095	3527941..3528477	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
IQS18_RS17100	3528530..3529759	+	1230	WP_203023910.1	benzoate/H(+) symporter BenE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3522276..3522779	503
	-	flank	IS/Tn	-	-	3529799..3530947	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T178594 WP_000813794.1 NZ_CP063511:c3525363-3525187 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T178594 NZ_CP084194:206967-207452 [Salmonella sp. A39]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT178594 WP_001270286.1 NZ_CP063511:c3525141-3524725 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT178594 NZ_CP084194:206692-206979 [Salmonella sp. A39]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 6HPB