Detailed information of TA system TA178583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2416138..2416763
• Replicon: chromosome
• Accession: NZ_CP063511
• Organism: Escherichia coli strain EH08-18-36

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IQS18_RS11650
• Protein ID: WP_000911330.1
• Coordinates: 2416365..2416763 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IQS18_RS11645
• Protein ID: WP_000450524.1
• Coordinates: 2416138..2416365 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS18_RS11620	2411940..2412410	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
IQS18_RS11625	2412410..2412982	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IQS18_RS11630	2413128..2414006	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IQS18_RS11635	2414023..2415057	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
IQS18_RS11640	2415270..2415983	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IQS18_RS11645	2416138..2416365	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IQS18_RS11650	2416365..2416763	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IQS18_RS11655	2416910..2417773	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
IQS18_RS11660	2417788..2419803	+	2016	WP_000829336.1	tRNA cytosine(34) acetyltransferase TmcA	-
IQS18_RS11665	2419877..2420575	+	699	WP_000679812.1	esterase	-
IQS18_RS11670	2420685..2420885	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T178583 WP_000911330.1 NZ_CP063511:2416365-2416763 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T178583 NZ_CP084168:c1546968-1546483 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT178583 WP_000450524.1 NZ_CP063511:2416138-2416365 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT178583 NZ_CP084168:c1547258-1546965 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33