Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1984927..1985581 Replicon chromosome
Accession NZ_CP063511
Organism Escherichia coli strain EH08-18-36

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IQS18_RS09600 Protein ID WP_000244777.1
Coordinates 1985174..1985581 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS18_RS09595 Protein ID WP_000354046.1
Coordinates 1984927..1985193 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS18_RS09575 1980896..1982329 - 1434 WP_001753034.1 6-phospho-beta-glucosidase BglA -
IQS18_RS09580 1982374..1982685 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IQS18_RS09585 1982849..1983508 + 660 WP_000250294.1 hemolysin III family protein -
IQS18_RS09590 1983704..1984684 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IQS18_RS09595 1984927..1985193 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS18_RS09600 1985174..1985581 + 408 WP_000244777.1 toxin CptA Toxin
IQS18_RS09605 1985621..1986142 - 522 WP_001055874.1 flavodoxin FldB -
IQS18_RS09610 1986254..1987150 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IQS18_RS09615 1987175..1987885 + 711 WP_001753033.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS18_RS09620 1987891..1989624 + 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T178581 WP_000244777.1 NZ_CP063511:1985174-1985581 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178581 NZ_CP084168:c318616-318514 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178581 WP_000354046.1 NZ_CP063511:1984927-1985193 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178581 NZ_CP084168:318507-318652 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References