Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 605407..605904 Replicon chromosome
Accession NZ_CP063511
Organism Escherichia coli strain EH08-18-36

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IQS18_RS03065 Protein ID WP_001173343.1
Coordinates 605407..605580 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IQS18_RS03070 Protein ID WP_000398619.1
Coordinates 605608..605904 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS18_RS03055 602077..603534 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
IQS18_RS03060 603771..605288 + 1518 WP_001295074.1 lysine--tRNA ligase -
IQS18_RS03065 605407..605580 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IQS18_RS03070 605608..605904 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IQS18_RS03075 606131..606403 - 273 WP_000405651.1 N-acetyltransferase -
IQS18_RS03080 606415..606645 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
IQS18_RS03085 606826..608457 + 1632 WP_001216477.1 sensor histidine kinase -
IQS18_RS03090 608454..609173 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 586702..605904 19202


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T178576 WP_001173343.1 NZ_CP063511:c605580-605407 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T178576 NZ_CP084109:c188539-188285 [Micrococcus luteus]
GTGCGCCTCGTGTGGGACCGCTCGGCCTGGGAGGACTACACGCACTGGCAGACCACGGACCGCAAGGTCCTCAAGCGGAT
CAACACCCTCATCGACGCCGCCCTGCGTGATCCCTTCGACGGGATCGGCAAGCCCGAGCAGCTGAAGTACGGCGCGCCCG
GGGCATGGTCCCGACGCATCACGGAGGAGCACAGGCTCGTCTACCTCGTCGACGGCGAGGACCTGATCATCCTCCAGGTG
AGGTATCACTACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT178576 WP_000398619.1 NZ_CP063511:c605904-605608 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT178576 NZ_CP084109:c188800-188540 [Micrococcus luteus]
ATGTCCATCAGTGCCAGTGAAGCCCGCAAGACCCTGTTCCCGCTCATCGAGCGAGTGAATGCGGACCGCGAGGTCGTGGA
GATCGTCTCCCGCAAGGGGAATGCCGTCCTCATGCCCGCGGACGAGTACGCCGCCTGGCAGGAGACCGCCTACCTGTTCC
GCTCCCCGGCCAACGCCCGCCGGCTGCTGGACGCCTACGAGCGCGCCCGCGCAGGGAGCGTCGAGGAGCACGCTCTGGAC
CGCATGGACGGGGACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References