Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 12533..13068 | Replicon | plasmid pEH09-18-41_3 |
| Accession | NZ_CP063506 | ||
| Organism | Escherichia coli strain EH09-18-41 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q3YTD6 |
| Locus tag | IQS19_RS23785 | Protein ID | WP_000222766.1 |
| Coordinates | 12781..13068 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0VIP2 |
| Locus tag | IQS19_RS23780 | Protein ID | WP_001132900.1 |
| Coordinates | 12533..12784 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IQS19_RS23745 | 7585..8328 | + | 744 | WP_039002216.1 | conjugal transfer pilus acetylase TraX | - |
| IQS19_RS23750 | 8383..8943 | + | 561 | WP_000139310.1 | fertility inhibition protein FinO | - |
| IQS19_RS23755 | 9078..9290 | + | 213 | WP_001541896.1 | ANR family transcriptional regulator | - |
| - | 9632..9693 | - | 62 | NuclAT_1 | - | - |
| - | 9632..9693 | - | 62 | NuclAT_1 | - | - |
| - | 9632..9693 | - | 62 | NuclAT_1 | - | - |
| - | 9632..9693 | - | 62 | NuclAT_1 | - | - |
| IQS19_RS23760 | 9680..9886 | + | 207 | WP_001351576.1 | type I toxin-antitoxin system Hok family toxin | - |
| IQS19_RS23765 | 10154..10411 | + | 258 | WP_001541895.1 | replication regulatory protein RepA | - |
| IQS19_RS23770 | 10643..10717 | + | 75 | WP_061357229.1 | RepA leader peptide Tap | - |
| IQS19_RS23775 | 10710..11567 | + | 858 | WP_039002220.1 | incFII family plasmid replication initiator RepA | - |
| IQS19_RS23780 | 12533..12784 | + | 252 | WP_001132900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| IQS19_RS23785 | 12781..13068 | + | 288 | WP_000222766.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| IQS19_RS23790 | 13143..13331 | - | 189 | Protein_11 | IS3 family transposase | - |
| IQS19_RS23795 | 13365..13865 | - | 501 | Protein_12 | Tn3 family transposase | - |
| IQS19_RS23800 | 13899..14945 | - | 1047 | Protein_13 | Tn3 family transposase | - |
| IQS19_RS23805 | 15120..15824 | + | 705 | WP_001067858.1 | IS6-like element IS26 family transposase | - |
| IQS19_RS23810 | 15878..16171 | + | 294 | Protein_15 | DDE-type integrase/transposase/recombinase | - |
| IQS19_RS23815 | 16220..16555 | - | 336 | Protein_16 | IS4 family transposase | - |
| IQS19_RS23820 | 16698..17558 | + | 861 | WP_000557454.1 | aminoglycoside N-acetyltransferase AAC(3)-IId | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aac(3)-IId / mcr-3.1 / blaTEM-1B / sul3 / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) | - | 1..131695 | 131695 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11147.23 Da Isoelectric Point: 10.5832
>T178563 WP_000222766.1 NZ_CP063506:12781-13068 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
Download Length: 288 bp
>T178563 NZ_CP084104:c114944-114633 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9211.41 Da Isoelectric Point: 4.0780
>AT178563 WP_001132900.1 NZ_CP063506:12533-12784 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT178563 NZ_CP084104:c114636-114217 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CDZ2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0VIP2 |