Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3958902..3959556 Replicon chromosome
Accession NZ_CP063503
Organism Escherichia coli strain EH09-18-41

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IQS19_RS18975 Protein ID WP_000244777.1
Coordinates 3958902..3959309 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS19_RS18980 Protein ID WP_000354046.1
Coordinates 3959290..3959556 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS19_RS18955 3954859..3956592 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
IQS19_RS18960 3956598..3957308 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS19_RS18965 3957333..3958229 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IQS19_RS18970 3958341..3958862 + 522 WP_001055874.1 flavodoxin FldB -
IQS19_RS18975 3958902..3959309 - 408 WP_000244777.1 toxin CptA Toxin
IQS19_RS18980 3959290..3959556 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS19_RS18985 3959799..3960779 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IQS19_RS18990 3960975..3961634 - 660 WP_000250274.1 hemolysin III family protein -
IQS19_RS18995 3961798..3962109 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IQS19_RS19000 3962154..3963587 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
IQS19_RS19005 3963644..3964387 - 744 WP_000951962.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T178554 WP_000244777.1 NZ_CP063503:c3959309-3958902 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178554 NZ_CP084103:1856257-1856359 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178554 WP_000354046.1 NZ_CP063503:c3959556-3959290 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178554 NZ_CP084103:c1856366-1856221 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References