Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1359965..1360583 Replicon chromosome
Accession NZ_CP063503
Organism Escherichia coli strain EH09-18-41

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IQS19_RS06495 Protein ID WP_001291435.1
Coordinates 1359965..1360183 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IQS19_RS06500 Protein ID WP_000344800.1
Coordinates 1360209..1360583 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS19_RS06460 1355254..1355826 + 573 WP_000779838.1 YbaY family lipoprotein -
IQS19_RS06465 1355857..1356168 - 312 WP_000409911.1 MGMT family protein -
IQS19_RS06475 1356547..1356900 + 354 WP_000878140.1 DUF1428 domain-containing protein -
IQS19_RS06480 1356942..1358492 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IQS19_RS06485 1358656..1359126 - 471 WP_000136192.1 YlaC family protein -
IQS19_RS06490 1359242..1359793 - 552 WP_000102564.1 maltose O-acetyltransferase -
IQS19_RS06495 1359965..1360183 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IQS19_RS06500 1360209..1360583 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IQS19_RS06505 1361129..1364278 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IQS19_RS06510 1364301..1365494 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178540 WP_001291435.1 NZ_CP063503:c1360183-1359965 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T178540 NZ_CP084096:c3594496-3594245 [Escherichia coli O78]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT178540 WP_000344800.1 NZ_CP063503:c1360583-1360209 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT178540 NZ_CP084096:c3594248-3593994 [Escherichia coli O78]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References