Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4481415..4481998 Replicon chromosome
Accession NZ_CP063499
Organism Escherichia coli strain EH10-18-47

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag IQS20_RS21525 Protein ID WP_000254738.1
Coordinates 4481663..4481998 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag IQS20_RS21520 Protein ID WP_096282549.1
Coordinates 4481415..4481663 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS20_RS21510 4477754..4479055 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
IQS20_RS21515 4479103..4481337 + 2235 WP_000226815.1 GTP pyrophosphokinase -
IQS20_RS21520 4481415..4481663 + 249 WP_096282549.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IQS20_RS21525 4481663..4481998 + 336 WP_000254738.1 endoribonuclease MazF Toxin
IQS20_RS21530 4482069..4482860 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IQS20_RS21535 4483088..4484725 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IQS20_RS21540 4484813..4486111 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T178533 WP_000254738.1 NZ_CP063499:4481663-4481998 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T178533 NZ_CP084096:c2821878-2821684 [Escherichia coli O78]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4665

>AT178533 WP_096282549.1 NZ_CP063499:4481415-4481663 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELINDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT178533 NZ_CP084096:2821507-2821685 [Escherichia coli O78]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure

References