Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4343959..4344613 Replicon chromosome
Accession NZ_CP063499
Organism Escherichia coli strain EH10-18-47

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IQS20_RS20895 Protein ID WP_000244777.1
Coordinates 4344206..4344613 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS20_RS20890 Protein ID WP_000354046.1
Coordinates 4343959..4344225 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS20_RS20865 4339128..4339871 + 744 WP_000951948.1 SDR family oxidoreductase -
IQS20_RS20870 4339928..4341361 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
IQS20_RS20875 4341406..4341717 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IQS20_RS20880 4341881..4342540 + 660 WP_000250274.1 hemolysin III family protein -
IQS20_RS20885 4342736..4343716 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IQS20_RS20890 4343959..4344225 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS20_RS20895 4344206..4344613 + 408 WP_000244777.1 toxin CptA Toxin
IQS20_RS20900 4344653..4345174 - 522 WP_001055874.1 flavodoxin FldB -
IQS20_RS20905 4345286..4346182 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IQS20_RS20910 4346207..4346917 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS20_RS20915 4346923..4348656 + 1734 WP_000813208.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T178532 WP_000244777.1 NZ_CP063499:4344206-4344613 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178532 NZ_CP084096:c2624953-2624846 [Escherichia coli O78]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGAGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178532 WP_000354046.1 NZ_CP063499:4343959-4344225 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178532 NZ_CP084096:2625000-2625066 [Escherichia coli O78]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References