Detailed information of TA system TA178528

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3476934..3477791
• Replicon: chromosome
• Accession: NZ_CP063499
• Organism: Escherichia coli strain EH10-18-47

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6YXS7
• Locus tag: IQS20_RS16705
• Protein ID: WP_046401692.1
• Coordinates: 3476934..3477314 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IQS20_RS16710
• Protein ID: WP_080343259.1
• Coordinates: 3477372..3477791 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS20_RS16685	3472183..3473106	-	924	WP_000535961.1	carboxylate/amino acid/amine transporter	-
IQS20_RS16690	3473217..3474401	-	1185	WP_001172876.1	sugar efflux transporter	-
IQS20_RS16695	3475286..3476137	-	852	WP_090050165.1	DUF4942 domain-containing protein	-
IQS20_RS16700	3476446..3476937	-	492	WP_046401693.1	hypothetical protein	-
IQS20_RS16705	3476934..3477314	-	381	WP_046401692.1	TA system toxin CbtA family protein	Toxin
IQS20_RS16710	3477372..3477791	-	420	WP_080343259.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IQS20_RS16715	3477760..3477981	-	222	WP_038633763.1	DUF987 domain-containing protein	-
IQS20_RS16720	3477993..3478478	-	486	WP_038642868.1	DNA repair protein RadC	-
IQS20_RS16725	3478531..3478926	-	396	WP_181825417.1	antirestriction protein	-
IQS20_RS16730	3479004..3479267	-	264	WP_001149870.1	hypothetical protein	-
IQS20_RS16735	3479267..3480085	-	819	WP_001234374.1	DUF945 domain-containing protein	-
IQS20_RS16740	3480201..3480451	-	251	Protein_3257	DUF905 family protein	-
IQS20_RS16745	3480642..3481109	-	468	WP_000727137.1	hypothetical protein	-
IQS20_RS16750	3481206..3481607	-	402	WP_000819262.1	hypothetical protein	-
IQS20_RS16755	3481813..3482661	-	849	WP_203161048.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (33-132)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14234.49 Da
• Isoelectric Point: 10.6224

>T178528 WP_046401692.1 NZ_CP063499:c3477314-3476934 [Escherichia coli]
MQTLSAIPTRKAPSRPTPVEIWQQLLTYLLKRHYGLSLSDTQFSDEKIITQYIDAGISLSDALNFLVEKSELVRIDRPGF
SIKHQSPFIGVIDILRARRATGLMQRNGYKRITLLIAGNAAQKQHS

Nucleotide

• Length: 381 bp

>T178528 NZ_CP084096:1296955-1297269 [Escherichia coli O78]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15460.52 Da
• Isoelectric Point: 7.4130

>AT178528 WP_080343259.1 NZ_CP063499:c3477791-3477372 [Escherichia coli]
MSFCAASPSVNSYSRSTTLLNKTLPANHHISEPWWGLKRNITPCFGARLVQEGNRLHYLADRASITGTFTDADLRHLDQA
FPALLKQLELMLVSGELNPRHQHCVTLYAKGLTCEADSLGSHGYIYTAIYPTPDDSITR

Nucleotide

• Length: 420 bp

>AT178528 NZ_CP084096:1296719-1296952 [Escherichia coli O78]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGATCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A7L6YXS7