Detailed information of TA system TA178512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 52888..53410
• Replicon: plasmid pEF5-18-41_4
• Accession: NZ_CP063496
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: IQS14_RS28285
• Protein ID: WP_000638823.1
• Coordinates: 52888..53169 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: IQS14_RS28290
• Protein ID: WP_000121741.1
• Coordinates: 53159..53410 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS28250	49037..49357	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
IQS14_RS28255	49428..49718	-	291	WP_032210188.1	conjugal transfer protein	-
IQS14_RS28260	49718..50302	-	585	WP_097728035.1	lytic transglycosylase domain-containing protein	-
IQS14_RS28265	50323..50721	-	399	WP_097728036.1	hypothetical protein	-
IQS14_RS28270	50840..51277	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
IQS14_RS28275	51283..52518	-	1236	WP_045146393.1	TcpQ domain-containing protein	-
IQS14_RS28280	52521..52820	-	300	WP_000835764.1	IncN plasmid KikA protein	-
IQS14_RS28285	52888..53169	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IQS14_RS28290	53159..53410	-	252	WP_000121741.1	hypothetical protein	Antitoxin
IQS14_RS28295	53510..54145	-	636	WP_015059536.1	hypothetical protein	-
IQS14_RS28300	54193..55025	+	833	Protein_70	DNA primase	-
IQS14_RS28305	55148..55402	-	255	WP_001043555.1	EexN family lipoprotein	-
IQS14_RS28310	55404..56045	-	642	WP_001463086.1	conjugal transfer protein TrbJ	-
IQS14_RS28315	56051..57046	-	996	WP_097728037.1	type IV secretion system protein	-
IQS14_RS28320	57050..57307	-	258	WP_000739144.1	hypothetical protein	-
IQS14_RS28325	57304..57606	-	303	WP_001360345.1	hypothetical protein	-
IQS14_RS28330	57622..57837	-	216	WP_021503156.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..63909	63909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T178512 WP_000638823.1 NZ_CP063496:c53169-52888 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T178512 NZ_CP084055:3430534-3430884 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT178512 WP_000121741.1 NZ_CP063496:c53410-53159 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT178512 NZ_CP084055:3430289-3430540 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1