Detailed information of TA system TA178511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 9514..10106
• Replicon: plasmid pEF5-18-41_4
• Accession: NZ_CP063496
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: IQS14_RS28020
• Protein ID: WP_106509039.1
• Coordinates: 9514..9762 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: IQS14_RS28025
• Protein ID: WP_000681609.1
• Coordinates: 9759..10106 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS27980	5058..5387	+	330	WP_000855119.1	hypothetical protein	-
IQS14_RS27985	5791..6306	+	516	WP_001025393.1	J domain-containing protein	-
IQS14_RS27990	6410..6952	+	543	WP_001083904.1	hypothetical protein	-
IQS14_RS27995	6949..7203	+	255	WP_001230709.1	hypothetical protein	-
IQS14_RS28000	7200..7442	+	243	WP_000151456.1	hypothetical protein	-
IQS14_RS28005	8013..8294	+	282	WP_021563355.1	hypothetical protein	-
IQS14_RS28010	8304..8720	+	417	WP_097728064.1	phage tail protein	-
IQS14_RS28015	8717..9028	+	312	WP_000627398.1	hypothetical protein	-
IQS14_RS28020	9514..9762	+	249	WP_106509039.1	type II toxin-antitoxin system HicA family toxin	Toxin
IQS14_RS28025	9759..10106	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IQS14_RS28030	10194..10376	-	183	WP_001428470.1	hypothetical protein	-
IQS14_RS28035	10934..11116	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
IQS14_RS28040	11141..11578	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
IQS14_RS28045	11709..12029	-	321	WP_001401701.1	hypothetical protein	-
IQS14_RS28050	12363..12541	-	179	Protein_20	hypothetical protein	-
IQS14_RS28055	12543..12746	-	204	WP_000224735.1	hypothetical protein	-
IQS14_RS28060	12752..13201	-	450	WP_052974092.1	hypothetical protein	-
IQS14_RS28065	13221..13928	-	708	WP_000108794.1	hypothetical protein	-
IQS14_RS28070	13930..14682	-	753	WP_000797966.1	conjugal transfer protein TraL	-
IQS14_RS28075	14700..15101	-	402	WP_021575837.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..63909	63909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9360.00 Da
• Isoelectric Point: 10.4800

>T178511 WP_106509039.1 NZ_CP063496:9514-9762 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILIHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T178511 NZ_CP084055:3326872-3327045 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT178511 WP_000681609.1 NZ_CP063496:9759-10106 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT178511 NZ_CP084055:3326548-3326844 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1I9ZJ49