Detailed information of TA system TA178509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 128912..129471
• Replicon: plasmid pEF5-18-41_2
• Accession: NZ_CP063494
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: IQS14_RS27455
• Protein ID: WP_000421257.1
• Coordinates: 128912..129187 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1M2FGI7
• Locus tag: IQS14_RS27460
• Protein ID: WP_072648935.1
• Coordinates: 129187..129471 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS27430	124216..125574	+	1359	WP_032353707.1	esterase-like activity of phytase family protein	-
IQS14_RS27435	125628..126354	-	727	Protein_140	IS256 family transposase	-
IQS14_RS27440	126453..126842	+	390	WP_001282144.1	IS66 family insertion sequence hypothetical protein	-
IQS14_RS27445	126839..127186	+	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
IQS14_RS27450	127236..128773	+	1538	Protein_143	IS66-like element ISEc8 family transposase	-
IQS14_RS27455	128912..129187	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IQS14_RS27460	129187..129471	-	285	WP_072648935.1	ribbon-helix-helix domain-containing protein	Antitoxin
IQS14_RS27465	129972..130562	+	591	WP_000086536.1	recombinase family protein	-
IQS14_RS27470	130609..130767	+	159	Protein_147	transposase	-
IQS14_RS27475	130917..131915	+	999	WP_032353474.1	ABC transporter substrate-binding protein	-
IQS14_RS27480	131915..132952	+	1038	WP_072674995.1	iron ABC transporter permease	-
IQS14_RS27485	132952..133713	+	762	WP_000020503.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..150711	150711
	-	inside	IScluster/Tn	-	-	120358..130562	10204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T178509 WP_000421257.1 NZ_CP063494:c129187-128912 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T178509 NZ_CP084055:c2227999-2227685 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10502.01 Da
• Isoelectric Point: 7.2042

>AT178509 WP_072648935.1 NZ_CP063494:c129471-129187 [Escherichia coli]
MKMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVKSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT178509 NZ_CP084055:c2227688-2227272 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A1M2FGI7