Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 118074..118599 Replicon plasmid pEF5-18-41_2
Accession NZ_CP063494
Organism Escherichia coli strain EF5-18-41

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IQS14_RS27385 Protein ID WP_001159871.1
Coordinates 118074..118379 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag IQS14_RS27390 Protein ID WP_000813630.1
Coordinates 118381..118599 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS14_RS27350 113204..113701 - 498 WP_072157241.1 tyrosine-type recombinase/integrase -
IQS14_RS27355 113878..114756 + 879 Protein_124 colicin-B -
IQS14_RS27360 114773..115470 - 698 Protein_125 IS1 family transposase -
IQS14_RS27365 115527..115631 - 105 Protein_126 colicin-B -
IQS14_RS27370 115873..116688 + 816 WP_000449473.1 lipid II-degrading bacteriocin colicin M -
IQS14_RS27375 116738..117091 - 354 WP_000864812.1 colicin M immunity protein -
IQS14_RS27380 117264..118073 - 810 WP_045145707.1 site-specific integrase -
IQS14_RS27385 118074..118379 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IQS14_RS27390 118381..118599 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IQS14_RS27395 119548..120245 - 698 Protein_132 IS1 family transposase -
IQS14_RS27400 120358..120489 + 132 Protein_133 transposase -
IQS14_RS27405 120537..120722 + 186 WP_032353630.1 hypothetical protein -
IQS14_RS27410 120754..121206 - 453 WP_032353631.1 N-acetyltransferase -
IQS14_RS27420 122848..123108 + 261 WP_032353703.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..150711 150711
- inside IScluster/Tn - - 120358..130562 10204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T178508 WP_001159871.1 NZ_CP063494:c118379-118074 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T178508 NZ_CP084055:c2161544-2161248 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT178508 WP_000813630.1 NZ_CP063494:c118599-118381 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT178508 NZ_CP084055:c2161246-2160851 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References