Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 73343..73986 Replicon plasmid pEF5-18-41_1
Accession NZ_CP063493
Organism Escherichia coli strain EF5-18-41

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag IQS14_RS26605 Protein ID WP_001034046.1
Coordinates 73570..73986 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag IQS14_RS26600 Protein ID WP_001261278.1
Coordinates 73343..73573 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS14_RS26570 68854..69159 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
IQS14_RS26575 69161..69379 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
IQS14_RS26580 69946..70458 + 513 WP_000151784.1 hypothetical protein -
IQS14_RS26585 70492..71625 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
IQS14_RS26590 71792..72565 - 774 WP_000905949.1 hypothetical protein -
IQS14_RS26595 72578..73078 - 501 WP_000528931.1 hypothetical protein -
IQS14_RS26600 73343..73573 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IQS14_RS26605 73570..73986 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
IQS14_RS26610 74031..77825 - 3795 WP_001144732.1 hypothetical protein -
IQS14_RS26615 78206..78436 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IQS14_RS26620 78433..78849 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / blaTEM-1B / sul3 / aac(3)-IId / sitABCD iutA / iucD / iucC / iucB / iucA 1..101982 101982


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T178505 WP_001034046.1 NZ_CP063493:73570-73986 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T178505 NZ_CP084055:c927349-927246 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT178505 WP_001261278.1 NZ_CP063493:73343-73573 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT178505 NZ_CP084055:927208-927479 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References