Detailed information of TA system TA178499

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4974735..4975567
• Replicon: chromosome
• Accession: NZ_CP063492
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: IQS14_RS24220
• Protein ID: WP_000854765.1
• Coordinates: 4975193..4975567 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: IQS14_RS24215
• Protein ID: WP_001295723.1
• Coordinates: 4974735..4975103 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS24185	4969928..4972048	+	2121	Protein_4724	autotransporter adhesin Ag43	-
IQS14_RS24190	4972119..4972277	+	159	WP_001323397.1	DUF905 family protein	-
IQS14_RS24195	4972432..4973250	+	819	WP_016231181.1	DUF945 domain-containing protein	-
IQS14_RS24200	4973305..4973790	+	486	WP_001735709.1	antirestriction protein	-
IQS14_RS24205	4973806..4974282	+	477	WP_001186725.1	RadC family protein	-
IQS14_RS24210	4974351..4974572	+	222	WP_001735710.1	DUF987 domain-containing protein	-
IQS14_RS24215	4974735..4975103	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IQS14_RS24220	4975193..4975567	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
IQS14_RS24225	4975564..4976000	+	437	Protein_4732	hypothetical protein	-
IQS14_RS24230	4976017..4976193	+	177	WP_000839288.1	DUF957 domain-containing protein	-
IQS14_RS24235	4976296..4976532	+	237	WP_001735712.1	hypothetical protein	-
IQS14_RS24240	4976555..4977775	-	1221	WP_001735713.1	capsular biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T178499 WP_000854765.1 NZ_CP063492:4975193-4975567 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T178499 NZ_CP084044:c4814348-4814064 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT178499 WP_001295723.1 NZ_CP063492:4974735-4975103 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT178499 NZ_CP084044:c4814580-4814338 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGTTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52