Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4794392..4795046 Replicon chromosome
Accession NZ_CP063492
Organism Escherichia coli strain EF5-18-41

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag IQS14_RS23235 Protein ID WP_000244769.1
Coordinates 4794392..4794799 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS14_RS23240 Protein ID WP_000354046.1
Coordinates 4794780..4795046 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS14_RS23215 4790349..4792082 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
IQS14_RS23220 4792088..4792798 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS14_RS23225 4792823..4793719 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
IQS14_RS23230 4793831..4794352 + 522 WP_001055874.1 flavodoxin FldB -
IQS14_RS23235 4794392..4794799 - 408 WP_000244769.1 protein YgfX Toxin
IQS14_RS23240 4794780..4795046 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS14_RS23245 4795289..4796269 + 981 WP_000886049.1 tRNA-modifying protein YgfZ -
IQS14_RS23250 4796346..4797005 - 660 WP_000250274.1 hemolysin III family protein -
IQS14_RS23255 4797169..4797480 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IQS14_RS23260 4797525..4798958 + 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T178497 WP_000244769.1 NZ_CP063492:c4794799-4794392 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178497 NZ_CP084044:4077582-4077800 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178497 WP_000354046.1 NZ_CP063492:c4795046-4794780 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178497 NZ_CP084044:4077181-4077555 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References