Detailed information of TA system TA178488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3219829..3221417
• Replicon: chromosome
• Accession: NZ_CP063492
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: IQS14_RS15590
• Protein ID: WP_001125415.1
• Coordinates: 3219829..3221151 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IQS14_RS15595
• Protein ID: WP_001296726.1
• Coordinates: 3221151..3221417 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS15550	3214863..3215435	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
IQS14_RS15555	3215586..3215777	-	192	WP_001469139.1	amidohydrolase family protein	-
IQS14_RS15560	3215728..3216147	-	420	WP_001322416.1	amidohydrolase family protein	-
IQS14_RS15565	3216107..3216262	-	156	WP_001322415.1	hypothetical protein	-
IQS14_RS15570	3216365..3217312	-	948	WP_000854796.1	aldo/keto reductase	-
IQS14_RS15575	3217386..3218075	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
IQS14_RS15580	3218084..3218392	-	309	WP_001221046.1	hypothetical protein	-
IQS14_RS15585	3218532..3219431	+	900	WP_001057776.1	LysR family transcriptional regulator	-
IQS14_RS15590	3219829..3221151	-	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
IQS14_RS15595	3221151..3221417	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IQS14_RS15600	3221628..3223028	-	1401	WP_001376867.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T178488 WP_001125415.1 NZ_CP063492:c3221151-3219829 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T178488 NZ_CP084032:4767459-4767746 [Pectobacterium colocasium]
ATGAGCTATAGCGTAAAATTCAGGGAAGATGCGCTAAAAGAATGGTGCAAGCTCGGTAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAGTTACGAGGAATGAAGGACTGCTACAAAATAA
AGCTCCGGGCGTCAGGCTTTCGTTTGGTTTACGAAGTCATTGACGACATATTGGTTATCGCCGTGGTGGCAGTAGGCAAA
CGTGAGCGCAGCGGCGTCTATAATCTGGCAAGTGAGCGGATGAGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT178488 WP_001296726.1 NZ_CP063492:c3221417-3221151 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT178488 NZ_CP084032:4767211-4767462 [Pectobacterium colocasium]
ATGCCAAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAACTGAAAAAGAACCCAATGGCGACGGTCAATGCTGG
CGATGGCCTACCTGTCGCTATCTTGAACCGCAATCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAGGAATTGGTTAAGCTAATCGGTGAACGTAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCCTATGA

Structures

Toxin

• AlphaFold DB: U9YVT8