Detailed information of TA system TA178486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3121044..3121682
• Replicon: chromosome
• Accession: NZ_CP063492
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: IQS14_RS15145
• Protein ID: WP_000813797.1
• Coordinates: 3121044..3121220 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IQS14_RS15150
• Protein ID: WP_076611057.1
• Coordinates: 3121266..3121682 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS15125	3116666..3117877	-	1212	WP_001323162.1	BenE family transporter YdcO	-
IQS14_RS15130	3117930..3118466	+	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
IQS14_RS15135	3118539..3120500	+	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IQS14_RS15140	3120592..3120822	-	231	WP_000494244.1	YncJ family protein	-
IQS14_RS15145	3121044..3121220	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IQS14_RS15150	3121266..3121682	+	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IQS14_RS15155	3121761..3123167	+	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
IQS14_RS15160	3123412..3124557	+	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
IQS14_RS15165	3124575..3125588	+	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
IQS14_RS15170	3125589..3126530	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T178486 WP_000813797.1 NZ_CP063492:3121044-3121220 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T178486 NZ_CP084032:c3943665-3943249 [Pectobacterium colocasium]
GTGAGCTGCTTGTTCATGCTGGATACCAATATCTGTTCCTTTATTATGCGTGAACACCCGGCGGCAGTGATTGATAAGTT
GCAGCAGTGCACGTTACGACGTGATCGAATTGTGGTCTCTGCCATCACTTACGCCGAAATGCGCTTTGGTGCGGTTGGCA
AAAAAGCTTCGCCACGCCATATGCAACTGGTTGATGCTTTCTGTCGTCGGCTCGATGCGGTGTTACCTTGGGATAGCGCC
GCGGTAGACGCGACGACGAAAATCAAAATTGCGTTAGCCGCGGTTGGTACACCCATTGGCCCAAATGATGCGGCCATCGC
CGGTCATGCCATCAGCGCTAAGGCCATATTGGTTACCAATAATCTGAGAGAGTTTGAGCGAGTACAGGGGCTAACGTTGG
AAGATTGGGTTAAATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT178486 WP_076611057.1 NZ_CP063492:3121266-3121682 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT178486 NZ_CP084032:c3943889-3943662 [Pectobacterium colocasium]
ATGCGTATTGTTTCGGTTTTTAAAAACGGTAACAACCGTGCTATTCGCTTGCCGCGTGATATGGATTTTGAGGGCGTCAA
CGAACTGGAGATCGTGCGTGAGGGGGATACCATCATGCTGCGTCCAGTGAAGCCCAATTGGCATTCATTTTTGCAAGAAG
AGCGCGCTGACGCTGACTTTATGACAGAACGTCAGGATGTGATTGAAGAGGGGAGATTCAAGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829J715