Detailed information of TA system TA178481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2722927..2723711
• Replicon: chromosome
• Accession: NZ_CP063492
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: IQS14_RS13000
• Protein ID: WP_000613626.1
• Coordinates: 2722927..2723421 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: IQS14_RS13005
• Protein ID: WP_001110447.1
• Coordinates: 2723418..2723711 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS12980	2718120..2719217	+	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-
IQS14_RS12985	2719217..2720158	+	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
IQS14_RS12990	2720224..2721867	+	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
IQS14_RS12995	2721879..2722832	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
IQS14_RS13000	2722927..2723421	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
IQS14_RS13005	2723418..2723711	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
IQS14_RS13010	2723844..2727029	-	3186	WP_000827427.1	ribonuclease E	-
IQS14_RS13015	2727602..2728561	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T178481 WP_000613626.1 NZ_CP063492:c2723421-2722927 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T178481 NZ_CP084032:798598-798972 [Pectobacterium colocasium]
ATGCAACTGTTGTCATTGGTTGTACATGGCCTCTTGGTATTGCTCATTCTTCTGGCGCCGTGGCCGGACGGCTATGCATG
GCTGTGGCTGTGCCTGGTCACGATGGTGATGTTCGGTTTTATCCGTAGCCAAAGGAATATTAAATCCCGACAGGGGGAAA
TCGTTCTGCTTAGCGAAACGACCCTGAACTGGCGGCAGCAGGAATGGCAGATCGTTAAACGACCGTGGTTGCTGAAAAAT
GGCGTGTTGCTGTCATTGCAGGCCGTTAACGGTAAAGACAGACAACAGCTTTGGCTGGCATCGGACAGCATGGGCGATGA
CGAGTGGCGTCACCTGCGTCAGCTTCTTTTGCAGCAGAAAAACTGGGCGAGATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT178481 WP_001110447.1 NZ_CP063492:c2723711-2723418 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT178481 NZ_CP084032:798312-798578 [Pectobacterium colocasium]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTATGACACACTCAGTGACAGCGATAAGCGCACGTTTGTACGCCTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCGAGCCGAAAGACGGAGAGCTGAAACGCATGATTTCTCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6