Detailed information of TA system TA178480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2474903..2475608
• Replicon: chromosome
• Accession: NZ_CP063492
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: IQS14_RS11825
• Protein ID: WP_000539521.1
• Coordinates: 2475222..2475608 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IQS14_RS11820
• Protein ID: WP_001280945.1
• Coordinates: 2474903..2475232 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS11805	2470060..2470971	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
IQS14_RS11810	2471149..2473497	+	2349	WP_023148675.1	EAL domain-containing protein	-
IQS14_RS11815	2473505..2474833	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
IQS14_RS11820	2474903..2475232	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
IQS14_RS11825	2475222..2475608	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IQS14_RS11830	2475834..2477159	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
IQS14_RS11835	2477372..2477755	+	384	WP_000497137.1	biofilm formation regulator BssR	-
IQS14_RS11840	2477866..2478981	+	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
IQS14_RS11845	2478978..2479604	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T178480 WP_000539521.1 NZ_CP063492:c2475608-2475222 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T178480 NZ_CP063492:c2475608-2475222 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT178480 WP_001280945.1 NZ_CP063492:c2475232-2474903 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT178480 NZ_CP063492:c2475232-2474903 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGACCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6