Detailed information of TA system TA178470

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1086983..1087807
• Replicon: chromosome
• Accession: NZ_CP063492
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2U2VR48
• Locus tag: IQS14_RS05145
• Protein ID: WP_053287712.1
• Coordinates: 1086983..1087360 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IQS14_RS05150
• Protein ID: WP_095454386.1
• Coordinates: 1087451..1087807 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS05105	1082221..1083618	+	1398	WP_001323091.1	PTS ascorbate transporter subunit IIC	-
IQS14_RS05110	1083634..1083939	+	306	WP_000218360.1	PTS ascorbate transporter subunit IIB	-
IQS14_RS05115	1083949..1084413	+	465	WP_000776521.1	PTS ascorbate transporter subunit IIA	-
IQS14_RS05120	1084427..1085077	+	651	WP_000056771.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
IQS14_RS05125	1085087..1085296	+	210	Protein_986	hypothetical protein	-
IQS14_RS05130	1085349..1086197	-	849	WP_053287709.1	DUF4942 domain-containing protein	-
IQS14_RS05135	1086281..1086478	-	198	WP_053287710.1	DUF957 domain-containing protein	-
IQS14_RS05140	1086498..1086986	-	489	WP_053287711.1	hypothetical protein	-
IQS14_RS05145	1086983..1087360	-	378	WP_053287712.1	TA system toxin CbtA family protein	Toxin
IQS14_RS05150	1087451..1087807	-	357	WP_095454386.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IQS14_RS05155	1087854..1088128	-	275	Protein_992	antitoxin of the YeeV-YeeU toxin-antitoxin system	-
IQS14_RS05160	1088501..1088722	-	222	WP_000692315.1	DUF987 domain-containing protein	-
IQS14_RS05165	1088785..1089261	-	477	WP_001349449.1	RadC family protein	-
IQS14_RS05170	1089277..1089756	-	480	WP_000860074.1	antirestriction protein	-
IQS14_RS05175	1089838..1090656	-	819	WP_053287871.1	DUF945 domain-containing protein	-
IQS14_RS05180	1090758..1090991	-	234	WP_053287870.1	DUF905 family protein	-
IQS14_RS05185	1090997..1091674	-	678	WP_001097362.1	hypothetical protein	-
IQS14_RS05190	1091793..1092677	-	885	WP_053287869.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (14-113)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14269.37 Da
• Isoelectric Point: 7.3006

>T178470 WP_053287712.1 NZ_CP063492:c1087360-1086983 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SPCTRSQLINSIDILRARRATGLMTRSHYRMVNNITLGKHCEAKQ

Nucleotide

• Length: 378 bp

>T178470 NZ_CP084031:3553654-3554976 [Aeromonas caviae]
ATGGCTAACCTTTGGGTATACATGAACGGTTACCGAGTGGGTACCTTTACCATGACAACTAGCGGTGCCCACCAATTTCA
GTACGATGAGTCTTGGGTAACACTGCCGGGTAGCCGCCCCATTTCTCTCTCTATGCCGTTACGGCAGCAGTGCTATCGAG
GCGATGAGCCCTATAACTTCTTCGGCAACTTGTTGCCGGATAATCTAGAAGTAAAAAACCGGGTGGTAGCACGTTACCAA
GCCAACTCAACCCAATCTTTTGATCTGCTCAGCTGCATTGGTCAGGACTGCGTAGGTGCATTGCAACTGGTGGCTCAGGG
TGCCCCTACGCCTGATGTGAAACGCATCGAATGTAAACCGCTCTCTGATGCTGAACTGGAGCATATCCTGACTAGTTACC
AACAAGGGATCCCTCTTGGGATGGTCAGGGAAGTTGAGGATTTCCGGATCTCTATCGCCGGGGCTCAGGAAAAGACCGCG
CTACTCTATCGTGACAACCGCTGGTACTTACCGAGTGCTGCGACCCCAACCACCCATATCATCAAGTTACCCATTGGTAA
GATCGAGAGCCACTCTTACTCGATAGATCTCTCCCAGAGCGTGGAGAACGAGTATCTCTGTACCCTGATAGCTAAAGAGT
TTGGTCTTCCCGTCCCGCATTGCTTCATTATGCAGGTAGGTAAGATCAAGGCTTTGGCAGTGGAGCGCTTCGATCGACGC
TACGCCGCTGATCGTAGCTGGATCATGCGTCTACCCCAAGAGGACTTCTGCCAAGTATTGAATGTACCGTCAGTACGGAA
GTACGAGAGCCATGGTGGGCCGAGTATTGCTGACATCATGACGACCCTGTTGGGGTCTGCCACACCGGAGCAGGATCGTT
ACCTGTTTATGAAGTCTCAAGTGCTGTTCTGGCTGCTGGCCGCGACTGACGGTCATGCAAAGAACTTCTCGGTCTTTATA
GAACCGGGGGGAAGCTTCCGACTGACACCATTTTACGACATTCTCTCTATGTATCCTGCATTTGGTGGTCGAGGGTTACA
TCCCCGAGATGCCAAACTGGCAATGGGACTTACCGCATCCAAGGGTAAGATATACGCGATAGACCATATATTTCCCTGCC
ATTTCTACCGTACAGCCAAAGCCGTTGGCTTTGAACAGGCGAAGATGAAACGGATTATCGCTGAGTTCATCGGCAATCTG
GATATGGTGATATCGAACGTTCGTGCGAAATTACCTGACACATTTCCCCCCCGAATTACGGAGTCCATCTTGAAAGGGCT
CACTACTAGGATCCAACGCCTTGATCTCTCCTCAATCGAGTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13280.07 Da
• Isoelectric Point: 6.6258

>AT178470 WP_095454386.1 NZ_CP063492:c1087807-1087451 [Escherichia coli]
IVSDTLSDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNP
RHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 357 bp

>AT178470 NZ_CP084031:3553400-3553654 [Aeromonas caviae]
ATGAAAGTAACCAGCGCGAAGCAGCTTAGTGCCTACCTCAAAGATGCCAGGCTTTTGCAAAAATATTCTCAGGGCAAGGT
TGCCGACAAGGTTGGCATCAGACAAGACACCGTCTCCAATTTTGAGCTCAATCCAGAATCAACCAAGTTGGATACCTTGT
TCAAAATCCTCGCCGCGTTGGAACTATCCCTTGATATCAAGCCTCGAACCGATCCACCGGATAGCCCTTCCTCAGGCTGG
AAAGAGGAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A2U2VR48