Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1015662..1016159 Replicon chromosome
Accession NZ_CP063492
Organism Escherichia coli strain EF5-18-41

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IQS14_RS04775 Protein ID WP_001173343.1
Coordinates 1015986..1016159 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IQS14_RS04770 Protein ID WP_000398619.1
Coordinates 1015662..1015958 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS14_RS04750 1012391..1013110 - 720 WP_000611288.1 two-component system response regulator DcuR -
IQS14_RS04755 1013107..1014738 - 1632 WP_001216477.1 sensor histidine kinase -
IQS14_RS04760 1014919..1015149 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
IQS14_RS04765 1015161..1015433 + 273 WP_000405647.1 N-acetyltransferase -
IQS14_RS04770 1015662..1015958 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IQS14_RS04775 1015986..1016159 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IQS14_RS04780 1016277..1017794 - 1518 WP_000003806.1 lysine--tRNA ligase -
IQS14_RS04785 1018031..1019488 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1015662..1025012 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T178469 WP_001173343.1 NZ_CP063492:1015986-1016159 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T178469 NZ_CP084031:c1598696-1597380 [Aeromonas caviae]
ATGATGGGTGGTTCTGAGCACCATCGGCTGGAACTTCGGCTGGGTAACCAGCTGATCGGCCATCTCGAATATGACTCCAG
CCGGGATGCGTTTCAGCTCGGCTATACACCTGAGTGGCAGGCGCGTGGCTTCCCGCTCTCCCCGCCGCTGGCGCTGGATG
GTTCGGCCAGCAGCGCCCAGATCCGGGCGTATCTCGGCAACTTGCTGCCTGAAAATCGCGGGCTGGAGCACCTGATTGAG
GCGCTCTCCGTTTCGCGCAGCAATCTCTTTGCCCTGATCCACGGCATCGGTCGCGATGTCTCAGGGGCGGTCACCTTTAC
CCTGCCCGCAGAGCCCGACGTCCCCACCCATTTTCGCCCCATCACCCCGAAAGAGCTGTTGCTGCGCCTCGATCAGCCCG
AGATCTGGCCGCTGGAAGTCTGGGATGGTCGCCCCCGCCTCTCGGTTGCGGGCGTGCAGGCCAAGCTCAACCTGCTGCGA
CTCGAGGGGACGCTGGGATTTGGCGAGGGCGAGCTCTGTTCCACCCACCTCCTCAAGTTCGAGCATCACCCTCATCTGGT
GATCAATGAGTTCGTGACCATGCGACTGGCCGCACTGCTGGGCATGCCGGTGGCGCGGGTCGAACTGCACAGGGTCGGCC
AAGGCGATAAGTCCCACCGAAGCTTGCTGGTGGAACGGTTCGATCGGCGCCTTGACGAGGATGGCTTGAGGGTGCGCCGT
CGTCACATGATCGATGCCTGTCAGGGGCTGGGGTTTCCCGTCGCCCGAAAATATGAACGCAACTTCGGTGACGGGCGGGA
CGTGGCCCATATTCGGGAAGGGGTGAGCCTGCCACGGCTGTTCAGTCTCAGCGCCCTCTGTGTCAATCCGGCGCAGGCCA
GACTGGACATGCTGCAATGGCTGCTCTTCAACCTCTGCGTCAACAACCACGATGCCCACGGCAAGAACTACAGCTTCTTC
GTATCAAAAGCAGGGCTGATGCCCACCCCCTGGTACGATCTGGTCAATGTGGGGCTTTACCCCGAGTTCAACCAGCATCT
GGCCATGGCCATAGGCGACGAGTTTGCTCCCGAGGCGGTCAACGCATGGCAGTTGCTGGAGATGGCCGAGGCATGCGAGC
TGCCCAAACGCCTGTTGCAGCAGACCTTGCACAAGATGGCCAGCGACATACTGCATCAGCTTGATGCCGCCATCGCCCTG
GTTGAGACAGACAATCCCGCCGAGCTGGCTTATCTGGCACGTTACAGAGACGACGTTGAGCACCGTTGCCACCATTTTCA
GGCGCAGGCGACGCTGTTGCCCGAGCTGCAACTGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT178469 WP_000398619.1 NZ_CP063492:1015662-1015958 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT178469 NZ_CP084031:c1598976-1598680 [Aeromonas caviae]
ATGATGGCAGACGCATCGTTGCAAACCGGCCCGAAAGCACTGGATCCCGCCTCCCTGGGGCTGGCGATCCGCGAGAGACG
CAAGGCCGCCAGGATGACGCAGGAGGTGACGGCCTCACTGTGCGGTATCTCCAAGAAGACCCTGATCAAGATTGAAAAAG
GGGGGGATGTCTACCTCTCGACCCTGTTGCAGGTGATGAAGGCGCTGGGGCTGCGCTTGCAACTGGTGCAGGAAATGGGC
TCACAGGTAGCGAGCGGCAACAGCCAACCCGAGGTCGGTGATGATGGGTGGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References