Detailed information of TA system TA178467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 346726..347338
• Replicon: chromosome
• Accession: NZ_CP063492
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: IQS14_RS01715
• Protein ID: WP_000833473.1
• Coordinates: 347153..347338 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: IQS14_RS01710
• Protein ID: WP_000499744.1
• Coordinates: 346726..347136 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS01695	342238..343053	-	816	WP_000891825.1	AraC family transcriptional regulator	-
IQS14_RS01700	343279..344679	+	1401	WP_000204802.1	MFS transporter	-
IQS14_RS01705	344690..346654	+	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
IQS14_RS01710	346726..347136	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IQS14_RS01715	347153..347338	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
IQS14_RS01720	347616..349154	-	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
IQS14_RS01725	349355..350506	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T178467 WP_000833473.1 NZ_CP063492:c347338-347153 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T178467 NZ_CP084027:c210211-209729 [Escherichia coli]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT178467 WP_000499744.1 NZ_CP063492:c347136-346726 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT178467 NZ_CP084027:c210465-210199 [Escherichia coli]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9