Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 17723..18315 Replicon plasmid pEF7-18-51_2
Accession NZ_CP063489
Organism Escherichia coli strain EF7-18-51

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag IQS15_RS22970 Protein ID WP_000520549.1
Coordinates 17723..17971 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag IQS15_RS22975 Protein ID WP_000681613.1
Coordinates 17968..18315 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS15_RS22940 14600..15115 + 516 WP_001025395.1 J domain-containing protein -
IQS15_RS22945 15220..15762 + 543 WP_001083906.1 hypothetical protein -
IQS15_RS22950 15759..16010 + 252 WP_001230707.1 hypothetical protein -
IQS15_RS22955 15997..16224 + 228 WP_000801440.1 hypothetical protein -
IQS15_RS22960 16678..16977 + 300 WP_000650309.1 hypothetical protein -
IQS15_RS22965 17068..17418 + 351 WP_000854259.1 hypothetical protein -
IQS15_RS22970 17723..17971 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
IQS15_RS22975 17968..18315 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IQS15_RS22980 18676..19179 + 504 WP_000781813.1 hypothetical protein -
IQS15_RS22985 19183..20412 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
IQS15_RS22990 20489..20782 + 294 WP_060598542.1 LuxR family transcriptional regulator -
IQS15_RS22995 20779..20994 + 216 WP_000760375.1 EexN family lipoprotein -
IQS15_RS23000 21350..21970 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
IQS15_RS23005 22038..22310 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T178466 WP_000520549.1 NZ_CP063489:17723-17971 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T178466 NZ_CP084027:c86004-85588 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT178466 WP_000681613.1 NZ_CP063489:17968-18315 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT178466 NZ_CP084027:c86231-86001 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References