Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 15759..16224 | Replicon | plasmid pEF7-18-51_2 |
| Accession | NZ_CP063489 | ||
| Organism | Escherichia coli strain EF7-18-51 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9ZUY2 |
| Locus tag | IQS15_RS22950 | Protein ID | WP_001230707.1 |
| Coordinates | 15759..16010 (+) | Length | 84 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | M7CCF8 |
| Locus tag | IQS15_RS22955 | Protein ID | WP_000801440.1 |
| Coordinates | 15997..16224 (+) | Length | 76 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IQS15_RS22925 | 11240..11767 | - | 528 | WP_000577043.1 | DUF2726 domain-containing protein | - |
| IQS15_RS22930 | 11902..12306 | - | 405 | WP_012421203.1 | hypothetical protein | - |
| IQS15_RS22935 | 12319..13185 | - | 867 | WP_001353743.1 | replication initiation protein | - |
| IQS15_RS22940 | 14600..15115 | + | 516 | WP_001025395.1 | J domain-containing protein | - |
| IQS15_RS22945 | 15220..15762 | + | 543 | WP_001083906.1 | hypothetical protein | - |
| IQS15_RS22950 | 15759..16010 | + | 252 | WP_001230707.1 | hypothetical protein | Toxin |
| IQS15_RS22955 | 15997..16224 | + | 228 | WP_000801440.1 | hypothetical protein | Antitoxin |
| IQS15_RS22960 | 16678..16977 | + | 300 | WP_000650309.1 | hypothetical protein | - |
| IQS15_RS22965 | 17068..17418 | + | 351 | WP_000854259.1 | hypothetical protein | - |
| IQS15_RS22970 | 17723..17971 | + | 249 | WP_000520549.1 | type II toxin-antitoxin system HicA family toxin | - |
| IQS15_RS22975 | 17968..18315 | + | 348 | WP_000681613.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| IQS15_RS22980 | 18676..19179 | + | 504 | WP_000781813.1 | hypothetical protein | - |
| IQS15_RS22985 | 19183..20412 | + | 1230 | WP_001329504.1 | relaxase/mobilization nuclease domain-containing protein | - |
| IQS15_RS22990 | 20489..20782 | + | 294 | WP_060598542.1 | LuxR family transcriptional regulator | - |
| IQS15_RS22995 | 20779..20994 | + | 216 | WP_000760375.1 | EexN family lipoprotein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mcr-1.1 | - | 1..33309 | 33309 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 84 a.a. Molecular weight: 9751.13 Da Isoelectric Point: 8.9187
>T178465 WP_001230707.1 NZ_CP063489:15759-16010 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
Download Length: 252 bp
>T178465 NZ_CP084027:39244-39714 [Escherichia coli]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA
Antitoxin
Download Length: 76 a.a. Molecular weight: 8659.98 Da Isoelectric Point: 8.5105
>AT178465 WP_000801440.1 NZ_CP063489:15997-16224 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
Download Length: 228 bp
>AT178465 NZ_CP084027:38804-39247 [Escherichia coli]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9ZUY2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Y6U8W9 |