Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 150754..151276 Replicon plasmid pEF7-18-51_1
Accession NZ_CP063488
Organism Escherichia coli strain EF7-18-51

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IQS15_RS22750 Protein ID WP_000220560.1
Coordinates 150995..151276 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IQS15_RS22745 Protein ID WP_000121743.1
Coordinates 150754..151005 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS15_RS22710 145951..146097 - 147 WP_059039682.1 hypothetical protein -
IQS15_RS22715 146164..146475 - 312 Protein_150 VRR-NUC domain-containing protein -
IQS15_RS22720 146550..146807 + 258 Protein_151 transposase -
IQS15_RS22725 146820..147971 - 1152 WP_202593114.1 IS30-like element IS30 family transposase -
IQS15_RS22730 148036..148284 - 249 Protein_153 transposase -
IQS15_RS22735 148345..148488 - 144 WP_152930453.1 hypothetical protein -
IQS15_RS22740 148648..149616 - 969 WP_152946993.1 IS5-like element IS903B family transposase -
IQS15_RS22745 150754..151005 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IQS15_RS22750 150995..151276 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IQS15_RS22755 151424..152404 - 981 WP_205595940.1 IS5 family transposase -
IQS15_RS22760 152667..154055 + 1389 WP_075699368.1 nitrate/nitrite transporter NarU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / blaTEM-1B / aac(3)-IId / dfrA15 / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / aph(3'')-Ib / aph(6)-Id - 1..172246 172246
- inside IScluster/Tn - - 144965..152404 7439


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T178464 WP_000220560.1 NZ_CP063488:150995-151276 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T178464 NZ_CP084026:c4220149-4219976 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT178464 WP_000121743.1 NZ_CP063488:150754-151005 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT178464 NZ_CP084026:c4220473-4220177 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References