Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 134173..134774 Replicon plasmid pEF7-18-51_1
Accession NZ_CP063488
Organism Escherichia coli strain EF7-18-51

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag IQS15_RS22660 Protein ID WP_001216034.1
Coordinates 134173..134553 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A826P616
Locus tag IQS15_RS22665 Protein ID WP_059039668.1
Coordinates 134553..134774 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS15_RS22635 129344..130438 - 1095 WP_059039567.1 hypothetical protein -
IQS15_RS22640 130456..132351 - 1896 Protein_135 Tn3 family transposase -
IQS15_RS22645 132404..133108 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
IQS15_RS22650 133173..133961 - 789 Protein_137 DUF968 domain-containing protein -
IQS15_RS22655 133989..134168 - 180 WP_038997082.1 hypothetical protein -
IQS15_RS22660 134173..134553 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IQS15_RS22665 134553..134774 - 222 WP_059039668.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IQS15_RS22670 134957..136513 + 1557 WP_038997084.1 type I restriction-modification system subunit M -
IQS15_RS22675 136510..137748 + 1239 WP_059039670.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / blaTEM-1B / aac(3)-IId / dfrA15 / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / aph(3'')-Ib / aph(6)-Id - 1..172246 172246


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T178463 WP_001216034.1 NZ_CP063488:c134553-134173 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T178463 NZ_CP084026:c4124365-4124015 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT178463 WP_059039668.1 NZ_CP063488:c134774-134553 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEIEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT178463 NZ_CP084026:c4124610-4124359 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A826P616

References