Detailed information of TA system TA178445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 1304343..1304938
• Replicon: chromosome
• Accession: NZ_CP063487
• Organism: Escherichia coli strain EF7-18-51

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: A0A827ISR3
• Locus tag: IQS15_RS06300
• Protein ID: WP_061359519.1
• Coordinates: 1304343..1304693 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: IQS15_RS06305
• Protein ID: WP_072711775.1
• Coordinates: 1304687..1304938 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS15_RS06280	1299792..1300814	-	1023	WP_001301928.1	ABC transporter permease	-
IQS15_RS06285	1300828..1302330	-	1503	WP_000205807.1	sugar ABC transporter ATP-binding protein	-
IQS15_RS06290	1302470..1303426	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
IQS15_RS06295	1303736..1304266	+	531	WP_000055070.1	inorganic diphosphatase	-
IQS15_RS06300	1304343..1304693	-	351	WP_061359519.1	endoribonuclease toxin ChpB	Toxin
IQS15_RS06305	1304687..1304938	-	252	WP_072711775.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
IQS15_RS06310	1305150..1305491	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
IQS15_RS06315	1305494..1309273	-	3780	WP_061359518.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12506.43 Da
• Isoelectric Point: 6.4786

>T178445 WP_061359519.1 NZ_CP063487:c1304693-1304343 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARQAKRIGLAADEVVEEALLRLQAVVK

Nucleotide

• Length: 351 bp

>T178445 NZ_CP084026:c965963-965634 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9283.58 Da
• Isoelectric Point: 4.6509

>AT178445 WP_072711775.1 NZ_CP063487:c1304938-1304687 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDINAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT178445 NZ_CP084026:c966301-965984 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A827ISR3