Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1233345..1233921 Replicon chromosome
Accession NZ_CP063487
Organism Escherichia coli strain EF7-18-51

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IQS15_RS05950 Protein ID WP_001297643.1
Coordinates 1233634..1233921 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag IQS15_RS05945 Protein ID WP_000063156.1
Coordinates 1233345..1233647 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS15_RS05930 1229941..1232091 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
IQS15_RS05935 1232141..1232344 + 204 WP_000467859.1 YbdD/YjiX family protein -
IQS15_RS05940 1232355..1233311 + 957 WP_001297640.1 GTPase -
IQS15_RS05945 1233345..1233647 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
IQS15_RS05950 1233634..1233921 - 288 WP_001297643.1 BrnT family toxin Toxin
IQS15_RS05955 1234120..1234817 - 698 Protein_1159 IS1 family transposase -
IQS15_RS05960 1234874..1234969 + 96 Protein_1160 dienelactone hydrolase family protein -
IQS15_RS05965 1235115..1236347 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
IQS15_RS05970 1236388..1237668 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T178444 WP_001297643.1 NZ_CP063487:c1233921-1233634 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T178444 NZ_CP084026:830987-831322 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT178444 WP_000063156.1 NZ_CP063487:c1233647-1233345 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT178444 NZ_CP084026:830739-830987 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References