Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 739121..739739 Replicon chromosome
Accession NZ_CP063487
Organism Escherichia coli strain EF7-18-51

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IQS15_RS03585 Protein ID WP_001291435.1
Coordinates 739521..739739 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IQS15_RS03580 Protein ID WP_000344800.1
Coordinates 739121..739495 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS15_RS03570 734210..735403 + 1194 WP_072711618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IQS15_RS03575 735426..738575 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IQS15_RS03580 739121..739495 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IQS15_RS03585 739521..739739 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IQS15_RS03590 739911..740462 + 552 WP_000102564.1 maltose O-acetyltransferase -
IQS15_RS03595 740578..741048 + 471 WP_000136192.1 YlaC family protein -
IQS15_RS03600 741212..742762 + 1551 WP_014639849.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IQS15_RS03605 742804..743157 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IQS15_RS03615 743536..743847 + 312 WP_000409911.1 MGMT family protein -
IQS15_RS03620 743878..744450 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178442 WP_001291435.1 NZ_CP063487:739521-739739 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T178442 NZ_CP084026:572196-572492 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT178442 WP_000344800.1 NZ_CP063487:739121-739495 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT178442 NZ_CP084026:572494-572889 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References