Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21336..21979 Replicon plasmid pEM03-18-08_3
Accession NZ_CP063486
Organism Escherichia coli strain EM03-18-08

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag IQS21_RS23695 Protein ID WP_001044768.1
Coordinates 21563..21979 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag IQS21_RS23690 Protein ID WP_001261287.1
Coordinates 21336..21566 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS21_RS23655 16527..16784 + 258 WP_001685860.1 hypothetical protein -
IQS21_RS23660 16786..17493 + 708 WP_058100829.1 hypothetical protein -
IQS21_RS23665 17497..17958 + 462 WP_024172415.1 hypothetical protein -
IQS21_RS23670 18264..18737 - 474 WP_058100828.1 hypothetical protein -
IQS21_RS23675 18914..19774 - 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -
IQS21_RS23680 20017..20721 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
IQS21_RS23685 20768..21040 - 273 WP_061602529.1 hypothetical protein -
IQS21_RS23690 21336..21566 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IQS21_RS23695 21563..21979 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
IQS21_RS23700 22141..24279 - 2139 WP_000350638.1 AAA family ATPase -
IQS21_RS23705 24633..24890 + 258 WP_000343085.1 hypothetical protein -
IQS21_RS23710 24890..25480 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / tet(A) - 1..71139 71139
- flank IS/Tn blaTEM-1B - 18914..20721 1807


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T178440 WP_001044768.1 NZ_CP063486:21563-21979 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T178440 NZ_CP084026:c463403-462939 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT178440 WP_001261287.1 NZ_CP063486:21336-21566 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT178440 NZ_CP084026:c463738-463403 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References