Detailed information of TA system TA178439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 15137..15684
• Replicon: plasmid pEM03-18-08_3
• Accession: NZ_CP063486
• Organism: Escherichia coli strain EM03-18-08

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: IQS21_RS23635
• Protein ID: WP_000969996.1
• Coordinates: 15403..15684 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A723YP93
• Locus tag: IQS21_RS23630
• Protein ID: WP_021559054.1
• Coordinates: 15137..15406 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS21_RS23595	10432..10848	-	417	WP_033548869.1	hypothetical protein	-
IQS21_RS23600	11381..11716	+	336	WP_001039462.1	hypothetical protein	-
IQS21_RS23605	11725..11916	+	192	WP_001515192.1	hypothetical protein	-
IQS21_RS23610	11927..12510	-	584	Protein_11	IS1 family transposase	-
IQS21_RS23615	13044..13799	+	756	WP_000807690.1	replication initiation protein RepE	-
IQS21_RS23620	14068..14413	+	346	Protein_13	IS1 family transposase	-
IQS21_RS23625	14924..15082	+	159	WP_072649723.1	hypothetical protein	-
IQS21_RS23630	15137..15406	+	270	WP_021559054.1	hypothetical protein	Antitoxin
IQS21_RS23635	15403..15684	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IQS21_RS23640	15719..15781	+	63	Protein_17	3'-5' exonuclease	-
IQS21_RS23645	15870..16154	+	285	WP_001407321.1	ribbon-helix-helix domain-containing protein	-
IQS21_RS23650	16154..16431	+	278	Protein_19	type II toxin-antitoxin system RelE/ParE family toxin	-
IQS21_RS23655	16527..16784	+	258	WP_001685860.1	hypothetical protein	-
IQS21_RS23660	16786..17493	+	708	WP_058100829.1	hypothetical protein	-
IQS21_RS23665	17497..17958	+	462	WP_024172415.1	hypothetical protein	-
IQS21_RS23670	18264..18737	-	474	WP_058100828.1	hypothetical protein	-
IQS21_RS23675	18914..19774	-	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / tet(A)	-	1..71139	71139
	-	flank	IS/Tn	blaTEM-1B	-	18914..20721	1807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T178439 WP_000969996.1 NZ_CP063486:15403-15684 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T178439 NZ_CP084023:4717333-4717620 [Pectobacterium aroidearum]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAACTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAGTTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCATCAGGCTTTCGTTTGGTTTATGAGGTTATTGACGACGTATTAATTATCGCCGTAGTGGCGGTAGGTAAA
CGTGAACGTAGTGGCGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10222.60 Da
• Isoelectric Point: 8.8735

>AT178439 WP_021559054.1 NZ_CP063486:15137-15406 [Escherichia coli]
MAQINMSLRIDAELKDAFMAAAKSMDRNASQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT178439 NZ_CP084023:4717085-4717336 [Pectobacterium aroidearum]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAATTGAAAAAGAACCCGATGGCGACGGTCAATGCTGG
CGACGGTCTACCTGTCGCTATTTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTTTACGAAAAAATGC
TGGATGCGCTGGACGACCAGGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCCTATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A723YP93