Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4710155..4710731 Replicon chromosome
Accession NZ_CP063483
Organism Escherichia coli strain EM03-18-08

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IQS21_RS22550 Protein ID WP_001297643.1
Coordinates 4710444..4710731 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag IQS21_RS22545 Protein ID WP_000063156.1
Coordinates 4710155..4710457 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS21_RS22530 4706751..4708901 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
IQS21_RS22535 4708951..4709154 + 204 WP_000467859.1 YbdD/YjiX family protein -
IQS21_RS22540 4709165..4710121 + 957 WP_001297640.1 GTPase -
IQS21_RS22545 4710155..4710457 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
IQS21_RS22550 4710444..4710731 - 288 WP_001297643.1 BrnT family toxin Toxin
IQS21_RS22555 4710930..4711627 - 698 Protein_4389 IS1 family transposase -
IQS21_RS22560 4711684..4711779 + 96 Protein_4390 dienelactone hydrolase family protein -
IQS21_RS22565 4711925..4713157 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
IQS21_RS22570 4713198..4714478 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T178434 WP_001297643.1 NZ_CP063483:c4710731-4710444 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T178434 NZ_CP084023:4177281-4178210 [Pectobacterium aroidearum]
ATGGATCAGCGTGATATTTATGCCCGACAGGTCAAGCTGTTAATGACCGCCCTGCCGTATGTGGCGAAAGAACCCTGTTT
CGCACTGAAAGGCGGCACCGCAATTAATTTATTTGTGCAGGATTTTCCTCGTCTATCGGTAGATATCGATCTGGTTTATC
AAACCTTCATAGATCGTGATACAGACCTAATCGCTATTGACGATGCATTGATGCGTATTACGGAATCGCTGAATGGCAGA
CAGGGGATCACAGCGATCCAGCAGGAGAATAAAGCCGATGAAAAACGCATCATCGTTAATACCGCTGATGCGCAGATAAA
GATTGAAGTCAGCCCGGTCTGGCGTGGACTGCTGTTGCCCCCAGCAGAAATGCCGGTATGTGAGCAGGTGGAAATGGAAT
ACGGCTTCACCACCATGAGCGTTGTCTCTCTGGCAGATCTTTACGGCGGTAAAATCTGCGCTGCGCTTGACCGCCAGCAT
CCGCGCGATCTGTTTGATGTACTGAATATGCTAGAAAAACCGTGCCTGACGCGTGAGATTTTTGATGGTTTTCTTTGCTA
TCTGGCAGGACATCCTCGCCCTATTGCCGAACTGCTGGCACCGAATTGGGACACATCGCGGATCGCCACACTATACGCCC
AGGAATTTTCAGGCATGACGCAGCAGGAAACCTCACTGGAATCGCTGTTATCCGTAGCGACTCTACTGCCGCAGGCGTTG
AAATCTCATTTCACCGCTCAGGACCGGCAATTCTTACTCAGCTATAAGCAAAACAACCCTGACTGGTCGCTGTATCGCTA
TCCTGATATTCAACATCTTCCCGCCATTCGCTGGAAACAGCGTAATTTATCGGCTTTACATGCTAAAAATCCGGCCAAAT
TTAGCGCGGCAGTAGGTAAGCTTGAGAAGGTTCTGGAACAACATTTTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT178434 WP_000063156.1 NZ_CP063483:c4710457-4710155 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT178434 NZ_CP084023:4176512-4177294 [Pectobacterium aroidearum]
TTGACAAATTATCTAAACTGGCTGATGCAAAACACCCTGCCCGGTCAGGTGTTGCTACAGTCATGGCTTACCCGCAACGG
TATAGACCGTAAGCTCTCTTACGTCTATGTCCAAAACGGTTGGCTCAGGCGGATCGCTCACGGCGTTTACTGCCGGACGG
GTCGTGAACCAGACTGGGTGGATGCGGTTTACTGCCTGCAAACTCAATGGGAAAAACCTGTGCGGGTAGCAGGGCTGACC
AGCCTTGCGCTACAGGGCTATTCCCATTATACCGAACTGGGTAAACCTCAAATTTGGCTCACGCTCCCGGCTTATATAAA
GCTGCCGGGATGGTTCACCGCCTTTAAACCATTCGCCACCTTTGTCGTGCTCTATACATCAGGCCTGTCGATTGATGTGA
TGCCATTCACGACAGAACTGAGAATCGGCGATCGGCGGCTGACTGGCAGCGTGCCAGAACTTGCCGCATATGAAATCGCC
AGCGGCGTTCCCGCACAAATATCGTTTGAGCATGCAGATGCATTGTTTCAGGGGCTGAACCTTCTCAGCCCGCGTAAATT
ACAGGCACTGCTCTATGCCAGCCATTCGGTAAAAACCAACCGGGTGATGCTCTACCTTGCCCAGCGCAATGGACATCCTT
ATTTTCAGTATCTCGATAAAAGTGGTATAGAAACAGGCGCTGGAAAGCGGCAAATTGTCCCCGGCGGCTGGCTGGAACCG
GATTACCAGATCACGGTTCCCAGGGAGTTTAAATCGGAAGGAGTCGAAGATGGATCAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References