Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4156187..4156805 Replicon chromosome
Accession NZ_CP063483
Organism Escherichia coli strain EM03-18-08

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IQS21_RS19940 Protein ID WP_001291435.1
Coordinates 4156587..4156805 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IQS21_RS19935 Protein ID WP_000344800.1
Coordinates 4156187..4156561 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS21_RS19925 4151276..4152469 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IQS21_RS19930 4152492..4155641 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IQS21_RS19935 4156187..4156561 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IQS21_RS19940 4156587..4156805 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IQS21_RS19945 4156977..4157528 + 552 WP_000102564.1 maltose O-acetyltransferase -
IQS21_RS19950 4157644..4158114 + 471 WP_000136192.1 YlaC family protein -
IQS21_RS19955 4158278..4159828 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IQS21_RS19960 4159870..4160223 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IQS21_RS19970 4160602..4160913 + 312 WP_000409911.1 MGMT family protein -
IQS21_RS19975 4160944..4161516 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178431 WP_001291435.1 NZ_CP063483:4156587-4156805 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T178431 NZ_CP084023:c3090509-3090195 [Pectobacterium aroidearum]
ATGCAATTCACCGTATACGGTAATACTGGGAAAAGCGTCGTTTACCCGCTACTGCTCGATGTCACGAGCGATATTATTGG
ACAATTGAATCGTCGGATAGTGATCCCATTACTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTGTCC
CCGTCGTCATGCTGACGGACGGCAAAGAATACGCGGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCAGGCCCTGGGG
GCGGTGTTTTGTGATGCTTCGCAGTACCGTTCTCAGGTAAAGGCTGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT178431 WP_000344800.1 NZ_CP063483:4156187-4156561 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT178431 NZ_CP084023:c3090748-3090512 [Pectobacterium aroidearum]
ATGACCGCGAAACAACGCAACACGCAGAGTGTGACCATGACAGTAGAGCGTGCCTTGCTGGTAAGAGCGCGTGAAGCGGG
TATTAACCTGAGTGCTACTCTGTCAACCGCGCTGGACGCGGAGCTTCGCCGTCATGAAGCGAAGAAATGGCAGGAAGAAA
ACAGGGAGGCTATCGACGCATTAAATCGTTTTCATGATGAAAACGGCTGTTTCAGTGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References